Protein, DNA, and virus crystallography with a focused imaging proportional counter.
Protein, DNA, and virus crystallography with a focused imaging proportional counter.
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使用聚焦成像正比计数器进行蛋白质、DNA 和病毒晶体学分析。
DOI：
10.1126/science.3704639
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Wiley,DC
中科院分区：
文献类型：
--
作者：
Durbin,RM;Burns,R;Moulai,J;Metcalf,P;Freymann,D;Blum,M;Anderson,JE;Harrison,SC;Wiley,DC
本文介绍了一套用商用二维聚焦几何电子探测器快速收集蛋白质和病毒晶体X射线强度数据的程序。该探测器结构紧凑，便于携带，具有与振荡摄影中使用的空间分辨率相当的高空间分辨率。它允许在具有大晶胞的弱衍射晶体上收集X射线数据，以及更传统的“衍射仪质量”晶体。的数据的质量进行了比较，从振荡摄影和自动衍射的范围内的单位细胞从96.3到383.2埃。同构和异常的差异Patterson，基于检测器数据，显示为一个可变的表面糖蛋白汞衍生物和阻遏物-DNA溴衍生物，已解决了在7埃与检测器数据。
A set of programs has been developed for rapid collection of x-ray intensity data from protein and virus crystals with a commercially available two-dimensional focused geometry electronic detector. The detector is compact and portable, with unusually high spatial resolution comparable to that used in oscillation photography. It has allowed x-ray data collection on weakly diffracting crystals with large unit cells, as well as more conventional "diffractometer-quality" crystals. The quality of the data is compared with that from oscillation photography and automated diffractometry in the range of unit cells from 96.3 to 383.2 angstroms. Isomorphous and anomalous difference Pattersons, based on detector data, are shown for a variable surface glycoprotein mercury derivative and for a repressor-DNA bromine derivative, which has been solved at 7 angstroms with detector data only.