Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes
Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes
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DOI：
10.1073/pnas.081288198
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发表时间：
2001-04-24
影响因子：
11.1
通讯作者：
Goodman, RM
中科院分区：
文献类型：
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作者：
Dasgupta, R;Garcia, BH;Goodman, RM
鸡舍病毒（FHV）是一种单链RNA昆虫病毒，以前曾报道过在大麦原生质体和几种植物的接种叶片中跨越界障并复制[sell， B， H, Allison， R. F. & Kaesberg， P, (1990) Proc. Natl.]。阿德莱德大学,科学。[j]。FHV在植物体内没有系统的运动。我们测试了植物病毒的运动蛋白（MPs）提供运动功能并引起FHV在植物中的全身传播的能力。我们比较了FHV在表达烟草花叶病毒或红三叶草坏死花叶病毒（RCNMV） MP的非转基因和转基因植株叶片中的生长情况。两种MPs都能在烟叶植物中调动FHV的细胞间和系统运动。转基因植株接种叶片中FHV的产率比非转基因植株接种叶片高100倍以上。此外，FHV在两株mp转基因植株未接种的上部叶片中均有积累。RCNMV MP在未接种的上叶中更有效地动员FHV。我们在这里也报道了FHV在另外六种植物的接种叶片中复制：苜蓿、拟南芥、芸苔、黄瓜、玉米和水稻。我们的研究结果表明，植物病毒MPs引起植物中动物病毒的细胞间和长距离运动，并为研究病毒的进化和植物中mRNA运输的控制机制以及开发有希望的外源基因在植物中瞬时表达的载体提供了方法。
Flock house virus (FHV), a single-stranded RNA insect virus, has previously been reported to cross the kingdom barrier and replicate in barley protoplasts and in inoculated leaves of several plant species [selling, B, H,, Allison, R. F. & Kaesberg, P, (1990) Proc. Natl. Acad, Sci. USA 87, 434-438]. There was no systemic movement of FHV in plants. We tested the ability of movement proteins (MPs) of plant viruses to provide movement functions and cause systemic spread of FHV in plants. We compared the growth of FHV in leaves of nontransgenic and transgenic plants expressing the MP of tobacco mosaic virus or red clover necrotic mosaic virus (RCNMV). Both MPs mobilized cell-to-cell and systemic movement of FHV in Nicotiana benthamiana plants. The yield of FHV was more than 100-fold higher in the inoculated leaves of transgenic plants than in the inoculated leaves of nontransgenic plants. In addition, FHV accumulated in the noninoculated upper leaves of both MP-transgenic plants. RCNMV MP was more efficient in mobilizing FHV to noninoculated upper leaves. We also report here that FHV replicates in inoculated leaves of six additional plant species: alfalfa, Arabidopsis, Brassica, cucumber, maize, and rice. Our results demonstrate that plant viral MPs cause cell-to-cell and long-distance movement of an animal virus in plants and offer approaches to the study of the evolution of viruses and mechanisms governing mRNA trafficking in plants as well as to the development of promising vectors for transient expression of foreign genes in plants.