The activation of Epimedium polysaccharide-propolis flavone liposome on Kupffer cells
The activation of Epimedium polysaccharide-propolis flavone liposome on Kupffer cells
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淫羊藿多糖-蜂胶黄酮脂质体对库普弗细胞的活化作用
DOI：
10.1016/j.carbpol.2015.07.044
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发表时间：
2015-11-20
影响因子：
11.2
通讯作者：
Song, Xiaoping
中科院分区：
文献类型：
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作者：
Fan, Yunpeng;Ren, Meimei;Song, Xiaoping
研究了淫羊藿多糖-淫羊藿黄酮脂质体（EPL）对枯否细胞（KCs）的体外免疫调节作用。结果表明，EPL能显着诱导趋化因子的分泌（RANTES和MCP-1），促进一氧化氮和诱导型一氧化氮合酶的产生，提高KC的吞饮和吞噬活性，促进TNF-α和IL-1 β的mRNA表达，并增强共刺激分子的表达（CD 11 B和CD 68）与淫羊藿多糖-淫羊藿黄酮（EP）相比，在30-7.5 μ g/mL。与EP相比，EPL刺激后KCs刺激淋巴细胞增殖和抗原提呈的能力明显增强。结果表明，EPL能激活KCs，具有较强的免疫调节作用，为进一步研究EPL提高免疫应答的机制提供了理论依据。(C)2015爱思唯尔有限公司版权所有。
Epimedium polysaccharide-propolis flavone liposome (EPL), a potent immunological pharmaceutical preparation, was investigated for the immunomodulatory activity on Kupffer cells (KCs) in vitro. The results showed that EPL could significantly induce the secretion of chemokines (RANTES and MCP-1), promote the production of nitric oxide and induced nitric oxide synthase, improve the pinocytic and phagocytic activity of KCs, promote the mRNA expression of TNF-alpha and IL-1 beta, and enhance the expression of costimulatory molecules (CD11 b and CD68) in KCs compared with Epimedium polysaccharide-propolis flavone (EP) at 30-7.5 mu g/mL. In addition, the abilities of KCs on stimulating lymphocytes proliferation and antigen presenting were significantly enhanced after stimulated with EPL compared with EP. These results suggested that EPL could activate KCs and possessed the stronger immunomodulatory effect, which provided the theoretical basis for further studying the mechanism of EPL on improving the immune response. (C) 2015 Elsevier Ltd. All rights reserved.