Salivary Glycine Is a Significant Predictor for the Attenuation of Polyp and Tumor Microenvironment Formation by Fucoxanthin in AOM/DSS Mice
Salivary Glycine Is a Significant Predictor for the Attenuation of Polyp and Tumor Microenvironment Formation by Fucoxanthin in AOM/DSS Mice
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DOI：
10.21873/invivo.11483
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发表时间：
2019-03
期刊：
影响因子：
2.3
通讯作者：
Masaru Terasaki;Saki Masaka;Chinami Fukada;Mayu Houzaki;T. Endo;Takuji Tanaka;Hayato Maeda;K. Miyashita;M. Mutoh
中科院分区：
文献类型：
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作者：
Masaru Terasaki;Saki Masaka;Chinami Fukada;Mayu Houzaki;T. Endo;Takuji Tanaka;Hayato Maeda;K. Miyashita;M. Mutoh
背景/目的：食用褐藻中富含岩藻黄素（Fx）这种高极性叶黄素，它在小鼠癌症模型中具有化学预防作用，但这些作用的潜在机制尚不清楚。因此，我们的目的是研究 Fx 对癌症模型小鼠肿瘤微环境的影响。材料和方法：我们研究了 Fx（30 毫克/千克体重）对 α 小鼠临床前结直肠癌模型的肿瘤微环境中多种细胞类型的影响，并分析了小鼠唾液，以寻找癌症化学预防作用的预测因子。结果：与未治疗的对照小鼠相比，Fx 给药显着减少了结直肠息肉的数量，并倾向于减少结肠病变。此外，与未治疗的对照小鼠相比，Fx 给药显示结直肠癌干细胞（如 CD44high/EpCAMhigh 细胞）、癌症相关成纤维细胞（如 αSMAhigh 细胞）、肿瘤相关巨噬细胞（如巨噬细胞）和树突状细胞（如 CD206high 细胞）的数量显着减少，分别为 0.6、0.5 和 0.6 倍。此外，治疗还显示唾液甘氨酸水平显着降低了 0.5 倍。结论：我们的结果表明唾液甘氨酸可能是代表 Fx 对小鼠化学预防作用的预测因子。
Background/ Aim: A high polar xanthophyll of Fucoxanthin (Fx) is abundantly contained in edible brown algae, and it has chemopreventive effects in mouse cancer models, however, the underlying mechanisms of these effects are not well understood. Thus, we aimed to investigate the effects of Fx on the tumor microenvironment in cancer model mice. Materials and Methods: We investigated the effect of Fx (30 mg/kg body weight) in a variety of cell types within the tumor microenvironment of α mouse preclinical colorectal cancer model and analyzed the mouse saliva in search of predictors for cancer chemopreventive effects. Results: Fx administration significantly decreased the number of colorectal polyps and tended to decrease colonic lesions compared to untreated control mice. In addition, Fx administration showed significantly lower numbers of colorectal cancer stem cells-like CD44high/EpCAMhigh cells, cancer-associated fibroblasts-like αSMAhigh cells, tumor-associated macrophages-like and dendritic cells-like CD206high cells by 0.6-, 0.5- and 0.6-fold, respectively, compared to untreated control mice. Moreover, the treatment also showed significantly lower levels of salivary glycine by 0.5-fold. Conclusion: Our results suggest that salivary glycine may be a predictor representing the chemopreventive effect of Fx in mice.