MALE-STERILITY INDUCTION IN TRANSGENIC TOBACCO PLANTS WITH AN UNEDITED ATP9 MITOCHONDRIAL GENE FROM WHEAT
MALE-STERILITY INDUCTION IN TRANSGENIC TOBACCO PLANTS WITH AN UNEDITED ATP9 MITOCHONDRIAL GENE FROM WHEAT
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DOI：
10.1073/pnas.90.6.2370
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发表时间：
1993-03-15
影响因子：
11.1
通讯作者：
MOURAS, A
中科院分区：
文献类型：
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作者：
HERNOULD, M;SUHARSONO, S;MOURAS, A
植物细胞质雄性不育与线粒体功能障碍有关。我们提出，一个由线粒体序列形成的核编码嵌合肽被导入线粒体后，可能损害细胞器功能并诱导植物雄性不育。本文报告了为验证这一假设而开发的一个模型。假设高等植物线粒体的编辑过程反映了产生活性蛋白的需求，我们将编辑过和未编辑过的小麦ATP合成酶亚基9 （atp9）编码序列融合到酵母coxIV转运肽的编码序列中。含有未编辑atp9的转基因植物表现出可育、半育或雄性不育的表型；含有编辑过的atp9或只有可选择标记的对照得到可育植株。可育植物花粉育性为31% ~ 75%，半可育植物为10% ~ 20%
Cytoplasmic male sterility in plants is associated with mitochondrial dysfunction. We have proposed that a nuclear-encoded chimeric peptide formed by mitochondrial sequences when imported into the mitochondria may impair organelle function and induce male sterility in plants. A model developed to test this hypothesis is reported here. Assuming that the editing process in higher plant mitochondria reflects a requirement for producing active proteins, we have used edited and unedited coding sequences of wheat ATP synthase subunit 9 (atp9) fused to the coding sequence of a yeast coxIV transit peptide. Transgenic plants containing unedited atp9 exhibited either fertile, semifertile, or male-sterile phenotypes; controls containing edited atp9 or only the selectable marker gave fertile plants. Pollen fertility ranged from 31% to 75% in fertile plants, 10% to 20% in semifertile plants, and