Use of Artificial Digestive Systems to Investigate the Biopharmaceutical Factors Influencing the Survival of Probiotic Yeast During Gastrointestinal Transit in Humans
Use of Artificial Digestive Systems to Investigate the Biopharmaceutical Factors Influencing the Survival of Probiotic Yeast During Gastrointestinal Transit in Humans
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DOI：
10.1007/s11095-011-0620-5
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发表时间：
2012-06-01
影响因子：
3.7
通讯作者：
Alric, Monique
中科院分区：
文献类型：
--
作者：
Blanquet-Diot, Stephanie;Denis, Sylvain;Alric, Monique
为了评价主要生物药剂学因素对新型益生酵母菌株CNCM I-3856在人工消化道中的存活率的影响，采用模拟人胃/小肠（TIM）和大肠（ARCOL）环境的动态系统，通过微生物计数法测定CNCM I-3856在整个人工消化道中的存活率。我们研究了盖伦制剂、食物摄入量、剂量、给药方式和频率对酵母菌存活率的影响。在禁食和进食状态下，与片剂相比，在羟丙基甲基纤维素（HPMC）胶囊中给予益生菌时，上消化道中的酵母菌活力显著更高。食物摄入导致酵母释放延迟和菌株存活率增加两倍。无论剂量如何，酵母菌对大肠环境特别敏感。高浓度的益生菌只能维持在结肠时，每天接种两次，超过5小时的时期。TIM和ARCOL是相关的筛选目的的互补工具，提供有价值的信息的影响盖伦形式，食物摄入量和剂量方案的益生菌在整个人体消化道的生存能力。
To evaluate the influence of the main biopharmaceutical factors on the viability of a new probiotic yeast strain, using dynamic systems simulating human gastric/small intestinal (TIM) and large intestinal (ARCOL) environments.The viability of CNCM I-3856 throughout the artificial digestive tract was determined by microbial counting. We investigated the effects of galenic formulation, food intake, dose, mode and frequency of administration on yeast survival rate.In both fasted and fed states, yeast viability in the upper digestive tract was significantly higher when the probiotic was administered in hydroxypropylmethylcellulose (HPMC) capsules compared to tablets. Food intake led to a delay in yeast release and a two-fold increase in strain survival. Whatever the dose, yeasts were particularly sensitive to the large intestinal environment. High concentrations of probiotic could only be maintained in the colon when it was inoculated twice a day over a 5-h-period.TIM and ARCOL are complementary tools relevant for screening purposes, supplying valuable information on the effects of galenic form, food intake and dose regimen on the viability of probiotics throughout the human digestive tract.