A ferrocene-switched electrochemiluminescence "off-on" strategy for the sensitive detection of cardiac troponin I based on target transduction and a DNA walking machine.
A ferrocene-switched electrochemiluminescence "off-on" strategy for the sensitive detection of cardiac troponin I based on target transduction and a DNA walking machine.
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DOI：
10.1039/c5cc04745e
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发表时间：
2015-09
影响因子：
4.9
通讯作者：
Ziqi Xu;Yongwang Dong;Jiyang Li;R. Yuan
中科院分区：
文献类型：
--
作者：
Ziqi Xu;Yongwang Dong;Jiyang Li;R. Yuan
基于靶点转导和DNA步行机原理，提出了一种二茂铁开关的电化学发光“关-开”传感器，用于心肌肌钙蛋白I（cTnI）的灵敏检测。所提出的方法提供了高选择性，低检测限为0.016 pg mL（-1），并提供了一个通用的平台，用于检测范围广泛的目标，包括其他蛋白质和DNA，通过改变分子识别元件相应的抗体，适配体或DNA探针。
A ferrocene-switched electrochemiluminescence "off-on" sensor is proposed on the basis of target transduction and a DNA walking machine for the sensitive detection of cardiac troponin I (cTnI). The proposed method offers high selectivity with a low detection limit of 0.016 pg mL(-1), and provides a universal platform for detecting a wide range of targets, including other proteins and DNAs, by changing the molecular recognition elements to the corresponding antibodies, aptamers or DNA probes.