Wounding tomato fruit elicits ripening-stage specific changes in gene expression and production of volatile compounds.
Wounding tomato fruit elicits ripening-stage specific changes in gene expression and production of volatile compounds.
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DOI：
10.1093/jxb/eru516
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发表时间：
2015-03
影响因子：
6.9
通讯作者：
Ferrante A
中科院分区：
文献类型：
--
作者：
Baldassarre V;Cabassi G;Spadafora ND;Aprile A;Müller CT;Rogers HJ;Ferrante A
随着番茄果实的成熟，损伤引发的转录组和VOC谱发生变化。这种转变与从保护发育中的种子到吸引食果动物传播种子的转变是一致的。肉质果实由一个未成熟的器官发育而来，需要保护其免受成熟器官的损害，成熟器官通过产生挥发性有机化合物（VOCs）吸引食果动物进行种子传播。因此，对伤害损伤的不同反应被预测。这项研究的目的是发现伤口诱导的转录组和VOC产生的变化是否会随着番茄从未成熟到成熟的转变而改变。采用微阵列分析方法，分析了两种商业沙拉番茄（Solanum lycopersicum L.）品种：Luna Rossa和AVG在伤害3h后的转录变化。随后采用定量PCR方法对红月露VOC生物合成和防御反应相关基因进行了检测。采用固相微萃取和气相色谱-质谱联用技术分析了不同成熟期损伤引起的挥发性有机化合物。在AVG中鉴定出约4000个差异表达基因，在红月中鉴定出2500个差异表达基因。在这两个品种中，大多数基因都被上调，受影响最大的途径是萜类、类胡萝卜素和脂质的代谢。防御相关基因大多在未成熟发育阶段上调，而VOCs相关基因的表达在成熟发育阶段发生变化。检测到40多种挥发性有机化合物，其分布随成熟期的变化而变化。因此，伤害引起的转录组和VOC谱都取决于成熟阶段，表明从防御到吸引的转变。
As tomato fruit ripen, the wounding-elicited transcriptome and VOC profiles alter. This shift is consistent with a change from protection of developing seeds to attraction of frugivores for seed dispersal. Fleshy fruits develop from an unripe organ that needs to be protected from damage to a ripe organ that attracts frugivores for seed dispersal through production of volatile organic compounds (VOCs). Thus, different responses to wounding damage are predicted. The aim of this study was to discover whether wound-induced changes in the transcriptome and VOC production alter as tomato transitions from unripe to ripe. Transcript changes were analysed 3h post-wounding using microarray analysis in two commercial salad-tomato (Solanum lycopersicum L.) cultivars: Luna Rossa and AVG, chosen for their high aroma production. This was followed by quantitative PCR on Luna Rossa genes involved in VOC biosynthesis and defence responses. VOCs elicited by wounding at different ripening stages were analysed by solid phase micro extraction and gas chromatography–mass spectrometry. Approximately 4000 differentially expressed genes were identified in the cultivar AVG and 2500 in Luna Rossa. In both cultivars the majority of genes were up-regulated and the most affected pathways were metabolism of terpenes, carotenoids, and lipids. Defence-related genes were mostly up-regulated in immature stages of development, whereas expression of genes related to VOCs changed at riper stages. More than 40 VOCs were detected and profiles changed with ripening stage. Thus, both transcriptome and VOC profiles elicited by wounding depend on stage of ripening, indicating a shift from defence to attraction.
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DOI：
10.21273/jashs.116.2.265
发表时间：
1991-03-01
影响因子：
1.9
作者：
BALDWIN, EA;NISPEROSCARRIEDO, MO;MOSHONAS, MG
通讯作者：
MOSHONAS, MG
影响因子：
7.2
作者：
Bate, NJ;Rothstein, SJ
通讯作者：
Rothstein, SJ
影响因子：
6.1
作者：
BUTTERY, RG;TERANISHI, R;LING, LC
通讯作者：
LING, LC
影响因子：
8.1
作者：
Farneti, Brian;Alarcon, Alberto Algarra;Woltering, Ernst J.
通讯作者：
Woltering, Ernst J.
影响因子：
7.4
作者：
Beekwilder, J;Alvarez-Huerta, M;Aharoni, A
通讯作者：
Aharoni, A