Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper
Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper
复制标题
DOI：
10.1002/ptr.1144
复制
发表时间：
2003-04-01
影响因子：
7.2
通讯作者：
Molnár, J
中科院分区：
文献类型：
--
作者：
Motohashi, N;Wakabayashi, H;Molnár, J
辣椒（Capsicum annuum L.）变种角叶木采用正己烷、丙酮、甲醇和70%甲醇4种溶剂对茄科茄属测定了提取物和组分的生物活性。这些提取物对两种人口腔肿瘤细胞系（HSC-2，HSG）比对正常人牙龈成纤维细胞（HGF）表现出相对较高的细胞毒活性，表明肿瘤特异性细胞毒活性。这些提取物的细胞毒活性通过硅胶[H2，A2，M1-M3]或ODS柱层析[70 M]分级分离而增强。几种组分[112，H4，H5，A1，A2，A3，A5，A6，A7，M2]逆转L5178小鼠淋巴瘤T细胞的多药耐药（MDR）表型，比（+/-）-维拉帕米更有效。提取物和组分未显示出任何可检测的抗人类免疫缺陷病毒（HIV）或抗幽门螺杆菌活性。因此，这项研究表明，有效的和选择性的抗肿瘤潜力甜椒'阿纳斯塔西娅红'进一步植物化学和生物学研究。版权所有（C）2003约翰威利父子有限公司。
The vegetable, Anastasia Red, Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ODS) column chromatography. The biological activities of extracts and fractions were determined. These extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (HSC-2, HSG) than against normal human gingival fibroblasts (HGF), suggesting a tumor-specific cytotoxic activity. The cytotoxic activity of these extracts was enhanced by fractionation on silica gel [H2, A2, M1-M3] or ODS column chromatography [70M]. Several fractions [112, H4, H5, A1, A2, A3, A5, A6, A7, M2] reversed the multidrug resistance (MDR) phenotype with L5178 mouse lymphoma T cells, more efficiently than (+/-)-verapamil. The extracts and fractions did not show any detectable anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. Thus, this study suggests the effective and selective antitumor potential of 'Anastasia Red' of sweet pepper for further phytochemical and biological investigation. Copyright (C) 2003 John Wiley Sons, Ltd.