Antioxidant Properties and Total Phenolic Content in Different Development Stages of Barringtonia racemosa and Barringtonia spicata Leaves
Antioxidant Properties and Total Phenolic Content in Different Development Stages of Barringtonia racemosa and Barringtonia spicata Leaves
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总状玉蕊和穗玉蕊不同发育阶段的抗氧化特性及总酚含量
DOI：
10.14456/867
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
A. A. Manaf
中科院分区：
文献类型：
--
作者：
Z. D. Dalila;J. Hafsah;A. A. Manaf
Barringtonia racemosa和Barringtonia spicata是中等大小的树木，可以在东南亚找到。它们是药用植物，在传统实践中用于治疗瘙痒，高血压，并通过使用它们的叶子作为净化剂。由于叶子的愈合特性，重要的是从叶子的不同发育阶段确定抗氧化特性和总酚含量。据推测，幼叶比成熟叶具有更高的活性。的总酚含量（TPC）的植物提取物一起使用1，1-二苯基-2-苦基肼（DPPH）测定的自由基清除能力，并通过使用硫氰酸铁（FTC）和硫代巴比妥（TBA）测定提取物抑制脂质过氧化的能力。在B的第1阶段叶的甲醇提取物中观察到最高的TPC。总状花序（0.34 mg GAE/g DW）和B.穗花（0.19 mg GAE/g DW）。B 3期叶片的甲醇提取物的清除活性最高。总状花序，IC 50为33.33 µg/ml。在B。与氯仿提取物相比，在第4阶段显示为42.33 µg/ml。所有提取物均能有效抑制脂质过氧化反应，抑制率均在88%以上. Barringtonia的愈合特性可能来自其高抗氧化活性。需要进行更详细的研究来确定叶子中存在哪些活性化合物作为抗氧化化合物。doi：10.14456/WJST.2015.84
Barringtonia racemosa and Barringtonia spicata are moderate sized trees that can be found in Southeast Asia. They are medicinal plants that have been used in traditional practice to treat itching, high blood pressure, and as a depurative, by using their leaves. Due to the healing properties of the leaves, it is important to determine the antioxidant properties and total phenolic content from the different developmental stages of the leaves. It was assumed that the young leaves have a higher activity than mature ones. The total phenolic content (TPC) of the plant extract was determined alongside the free radical scavenging ability using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, and also the capacity of the extracts to inhibit lipid peroxidation by using a ferric thiocyanate (FTC) and thiobarbituric (TBA) assay. The highest TPC was observed in the methanolic extracts of the stage 1 leaves of B. racemosa (0.34 mg GAE/g DW) and B. spicata (0.19 mg GAE/g DW). The highest scavenging activity was shown in the methanolic extract from the stage 3 leaf of B. racemosa , with IC 50 at 33.33 µg/ml. In B. spicata , it was shown in stage 4, at 42.33 µg/ml, as compared to the chloroform extraction. All extracts actively inhibited lipid peroxidation with an amount of more than 88 % inhibition. The healing properties of Barringtonia could come from their high antioxidant activities. More detailed study will be required to identify which of the active compounds are present in the leaves that act as antioxidant compounds. doi: 10.14456/WJST.2015.84