Integrin α3β1 suppresses long-term potentiation at inhibitory synapses on the cerebellar Purkinje neuron
Integrin α3β1 suppresses long-term potentiation at inhibitory synapses on the cerebellar Purkinje neuron
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DOI：
10.1016/j.mcn.2005.10.012
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Hirano, T
中科院分区：
文献类型：
--
作者：
Kawaguchi, SY;Hirano, T
在小脑中抑制性中间神经元和浦肯野神经元（PN）之间的GABA能突触处，突触后去极化诱导GABA（A）受体反应性的长时程增强（称为反弹增强; RP）。在这里，我们表明，整合素，一种类型的细胞粘附分子，参与RP的调节。Mn ~（2+）激活整合素可抑制RP诱导的PN中GABA反应性和mIPSC，而针对整合素α 3或β 1亚基的功能阻断抗体可阻断该效应，但针对整合素α 5或α V亚基的功能阻断抗体则不能阻断该效应。此外，PN中整合素α 3亚基的过度表达本身就损害了RP的诱导。我们还表明，Src家族的蛋白酪氨酸激酶介导的整合素活性的RP诱导的抑制作用。因此，整合素/Src途径负调控诱导的长期可塑性在抑制性突触小脑PN。(c)2005年爱思唯尔公司All rights reserved.
At the GABAergic synapses between inhibitory interneurons and a Purkinje neuron (PN) in the cerebellum, the postsynaptic depolarization induces the long-term potentiation (called rebound potentiation; RP) of GABA(A) receptor responsiveness. Here, we show that integrins, a type of cell-adhesion molecules, are involved in the regulation of RP. Integrin activation by Mn2+ impaired the RP induction of GABA responsiveness and mIPSCs in PNs, which was abolished by the function blocking antibody against either integrin alpha 3 or beta 1 subunit, but not by that against alpha 5 or alpha V subunit. Furthermore, overexpression of integrin alpha 3 subunit in a PN by itself impaired the RP induction. We also show that Src-family of protein tyrosine kinases mediated the suppressive effect of integrin activity on the RP induction. Thus, the integrin/Src pathway negatively regulates the induction of long-term plasticity at inhibitory synapses on a cerebellar PN. (c) 2005 Elsevier Inc. All rights reserved.