VH gene segments in the mouse and human genomes
VH gene segments in the mouse and human genomes
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DOI：
10.1016/j.jmb.2004.06.055
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发表时间：
2004-09-03
影响因子：
5.6
通讯作者：
Chothia, C
中科院分区：
文献类型：
--
作者：
de Bono, B;Madera, M;Chothia, C
我们已经检查了小鼠基因组序列，以确定其V-H基因片段库。总共有141个片段被定位到12号染色体的3 Mb区域。有证据表明，其中92个在用于基因组序列的小鼠品系C57 BL/6 J中有功能; 12个在其他小鼠品系中有功能，37个是假基因。因此，小鼠V-H基因片段库的大小是人类的两倍。小鼠和人类的基因座彼此没有大规模的相似性。104个功能片段属于15个已知序列亚组之一，在此进一步聚类为8组。这些集合中的7个，包括101个序列，与5个人类V-H家族相关，并且在它们的高变区中具有相同的典型结构。在基因座的远侧一半中的一组成员的复制主要是负责小鼠库的较大尺寸。V-H片段的系统发育分析表明，人类和小鼠V-H基因座中的大多数序列是在这两种生物体从它们的共同祖先分化之后出现的。(C)2004爱思唯尔有限公司保留所有权利。
We have examined the mouse genome sequence to determine its V-H gene segment repertoire. In all, 141 segments are mapped to a 3 Mb region of chromosome 12. There is evidence that 92 of these are functional in the mouse strain used for the genome sequence, C57BL/6J; 12 are functional in other mouse strains, and 37 are pseudogenes. The mouse V-H gene segment repertoire is therefore twice the size of that in humans. The mouse and human loci bear no large-scale similarity to each other. The 104 functional segments belong to one of the 15 known sequence subgroups, which have been further clustered into eight sets here. Seven of these sets, comprising 101 sequences, are related to five of the human V-H families and have the same canonical structures in their hypervariable regions. Duplication of members of one set in the distal half of the locus is mainly responsible for the larger size of the mouse repertoire. Phylogenetic analysis of the V-H segments indicates that most of the sequences in the human and mouse V-H loci have arisen subsequent to the divergence of the two organisms from their common ancestor. (C) 2004 Elsevier Ltd. All rights reserved.