Quencher-free, end-stacking oligonucleotides for probing single-base mismatches in DNA
Quencher-free, end-stacking oligonucleotides for probing single-base mismatches in DNA
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DOI：
10.1021/ol0518582
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发表时间：
2005-10-27
期刊：
影响因子：
5.2
通讯作者：
Kim, BH
中科院分区：
文献类型：
--
作者：
Seo, YJ;Ryu, JH;Kim, BH
[图形]荧光可以通过PET从荧光团猝灭到相邻的C、T和G碱基，但不能猝灭到A部分。由π堆积引起的发夹茎稳定性和芘标记的2 '-脱氧核苷酸单元与其相邻碱基之间的PET是影响这些新型荧光寡核苷酸的操作的两个主要因素。
[GRAPHICS]Fluorescence can be quenched through PET from the fluorophore to neighboring C, T, and G bases, but not to the A moiety. The hairpin stem stability arising from :pi-stacking and the PET between the pyrene-labeled 2'-deoxynucleotide units and their neighboring bases are the two main factors that affect the operation of these novel fluorescent oligonucleotides.