AUTORADIOGRAPHIC ANALYSIS OF ODONTOBLAST REPLACEMENT FOLLOWING PULP EXPOSURE IN PRIMATE TEETH
AUTORADIOGRAPHIC ANALYSIS OF ODONTOBLAST REPLACEMENT FOLLOWING PULP EXPOSURE IN PRIMATE TEETH
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DOI：
10.1016/0003-9969(90)90093-p
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发表时间：
1990-01-01
影响因子：
3
通讯作者：
HEYS, DR
中科院分区：
文献类型：
--
作者：
FITZGERALD, M;CHIEGO, DJ;HEYS, DR
研究了灵长类动物牙齿牙髓暴露后不久发生的成牙本质细胞替换相关的细胞迁移和复制。导致牙髓暴露的5类洞准备用含氢氧化钙的封闭剂和汞合金修复。在此之后84和96小时，给动物注射0.5 μ Ci/g体重氚化胸苷（sp. act. 6.7 Ci/mM）。分别于治疗后6、8、10、12天拔牙。在三个60 × 106细胞中计数成牙本质细胞样、成纤维细胞样和血管周围细胞的标记细胞数以及每个标记细胞的巨细胞数。260 μ m区域。这些区域代表正常牙髓组织的成牙本质细胞和无细胞（1区）、细胞丰富（2区）和深髓（3区）区域。对每颗牙齿计数以暴露中点为中心的10个切片。早在第8天，在盖顶剂和牙髓之间的界面处观察到基质形成和标记的成牙本质细胞样细胞。 其他重要发现包括：（1）随着时间的推移，1区标记的成牙本质细胞样细胞增加，表明分化细胞的持续流入;（2）区1中标记细胞随时间增加，同时区3中标记细胞减少，这表明区1中细胞的流入来自较深的牙髓1，和（3）区、处理时间和细胞类型之间颗粒计数的差异，表明在初始处理和最终成牙本质细胞样细胞分化之间至少发生了两次DNA复制。
Cell migration and replication associated with odontoblast replacement occurring soon after pulp exposure in primate teeth were studied. Class 5 cavity preparations resulting in pulp exposures were restored with a calcium hydroxide-containing capping agent and amalgam. Eighty-four and 96 h after this the animals were injected with 0.5 .mu.Ci/g body wt tritiated thymidine (sp. act. 6.7 Ci/mM). Teeth were extracted 6, 8, 10 and 12 days after treatment. The number of labelled cells as well as the number of giants per labelled cell were counted for odontoblast-like, fibroblast-like and perivascular cells in three 60 .times. 260 .mu.m zones. These zones represented the odontoblast and cell-free (zone 1), cell-rich (zone 2) and deep pulp (zone 3) areas of normal pulp tissue. Ten sections centred around the mid-point of the exposure were counted for each tooth. Matrix formation and labelled odontoblast-like cells were observed at the interface between the capping agent and the pulp as early as day 8. Other significant findings were: (1) an increase in labelled odontoblast-like cells in zone 1 over time, suggesting a continual influx of differentiating cells; (2) an increase in labelled cells in zone 1 over time with a concurrent decrease in zone 3, suggesting that he influx of cells in zone 1 was from the deeper pulpl and (3) differences in grain counts between zones, treatment times and cell types, indicating that at least two DNA replication had occurred between initial treatment and final odontoblast-like cell differentiation.