Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells.
Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells.
复制标题
血脑屏障内皮细胞跨内皮电阻的测量。
DOI：
10.1007/978-1-0716-3429-5_16
复制
发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Tharakan,Binu
中科院分区：
文献类型：
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作者：
Waithe,O'lisaYaa;Peng,Xu;Childs,EdW;Tharakan,Binu
血脑屏障（BBB）是大脑的保护屏障，其完整性是维持正常微血管通透性和大脑稳态的关键。脑微血管内皮细胞是血脑屏障的主要组成部分。跨内皮电阻（TEER）是跨细胞单层（如脑微血管内皮细胞单层）的电阻。TEER的测量被认为是一种敏感、可靠和非侵入性的方法，用于评价体外条件下内皮细胞单层的屏障完整性和渗透性。TEER的测量也有助于研究各种细胞和分子变化以及调节内皮单层屏障功能的信号事件。通常用于测量TEER的体外内皮细胞屏障模型之一是使用单层生长的人或啮齿动物脑微血管内皮细胞的BBB模型。在本协议中，我们描述了如何TEER是测量脑微血管内皮细胞单层，以确定血脑屏障的完整性在体外条件下。
The integrity of the blood-brain barrier (BBB), the protective barrier of the brain, is key to maintaining normal microvascular permeability and brain homeostasis. Brain microvascular endothelial cells are primary components of the blood-brain barrier. Transendothelial electrical resistance (TEER) is the electrical resistance across a cellular monolayer such as the brain microvascular endothelial cell monolayers. Measurement of TEER is considered a sensitive, reliable, and noninvasive method for evaluating barrier integrity and permeability of an endothelial cell monolayer under in vitro conditions. Measurement of TEER is also helpful for studying various cellular and molecular changes and signaling events that regulate barrier functions in endothelial monolayers. One of the in vitro endothelial cell barrier models that have been commonly used for measuring TEER is the BBB model using human or rodent brain microvascular endothelial cells grown as a monolayer. In this protocol, we describe how TEER is measured in brain microvascular endothelial cell monolayers, to determine blood-brain barrier integrity under in vitro conditions.