THE PRIMARY STRUCTURE OF A PLANT-STORAGE PROTEIN - ZEIN
THE PRIMARY STRUCTURE OF A PLANT-STORAGE PROTEIN - ZEIN
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DOI：
10.1093/nar/9.19.5163
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
MESSING, J
中科院分区：
文献类型：
--
作者：
GERAGHTY, D;PEIFER, MA;MESSING, J
玉米醇溶蛋白（玉米的主要贮藏蛋白）的代表性蛋白质序列已衍生自玉米醇溶蛋白cDNA克隆的核苷酸序列。用Maxam和吉尔伯特及M_（13）-双脱氧技术测定了该cDNA的序列。核苷酸序列包括mRNA的非翻译3′端、指定成熟玉米醇溶蛋白和小信号肽的整个编码序列以及非翻译5′区的一部分。推导的氨基酸组成和氨基末端的氨基酸序列非常相似，来自玉米醇溶蛋白的蛋白质馏分的化学分析。所提供的数据代表了植物贮藏蛋白的第一个完整氨基酸序列。
The protein sequence of a representative of the zeins, the major storage proteins of maize, has been derived from the nucleotide sequence of a zein cDNA clone. This cDNA was sequenced both by the Maxam and Gilbert and the M13-dideoxy techniques. The nucleotide sequence encompasses the non-translated 3′ terminus of the mRNA, the entire coding sequence specifying both the mature zein protein and a small signal peptide, and a portion of the non-translated 5′ region. The deduced amino acid composition and the amino-terminal amino acid sequence closely resemble those derived from chemical analysis of the zein protein fraction. The data presented represent the first complete amino acid sequence of a plant storage protein.