Genotypic and phenotypic evidence of different drug-resistance mutation patterns between B and non-B subtype isolates of human immunodeficiency virus type 1 found in Brazilian patients failing HAART
Genotypic and phenotypic evidence of different drug-resistance mutation patterns between B and non-B subtype isolates of human immunodeficiency virus type 1 found in Brazilian patients failing HAART
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DOI：
10.1023/a:1011812810397
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发表时间：
2001-01-01
期刊：
影响因子：
1.6
通讯作者：
Tanuri, A
中科院分区：
文献类型：
--
作者：
Caride, E;Hertogs, K;Tanuri, A
我们研究了从HAART治疗失败的个体中分离的14株HIV-1菌株对蛋白酶抑制剂（PI）的表型和基因型易感性。对原病毒和血浆病毒pol基因片段进行扩增、测序和分型。9份样本聚集有蛋白酶B亚型参考菌株，其余样本为非B亚型，分别为F亚型（n=4）和A亚型（n=1）。虽然所有患者均采用类似的PI药物方案治疗，但非b亚型分离株未出现L90M和I84V突变，主要使用G48V和V82A/F实现耐药。所有4个PI的强交叉抗性表型与b亚型分离株的L90M突变相关，与非b亚型分离株的G48V和V82A \F突变相关。这一观察结果表明，与b亚型分离株相比，非b型病毒具有特定的基因型特征。
We have investigated the phenotypic and genotypic susceptibility of 14 HIV-1 strains isolated from individuals failing HAART therapy to protease inhibitors (PI). Proviral and plasma viral pol gene fragment were amplified, sequenced and subtyped. Nine samples clustered with protease subtype B reference strains and the remaining samples were classified as non-B subtype corresponding to subtype F (n=4) and subtype A (n=1). Although all patients were treated with similar PI drug regimen, the non-B subtype isolates did not present the L90M and I84V mutations and used mainly G48V and V82A/F to achieve drug resistance. A strong cross-resistance phenotype among all four PI was associated with the mutation L90M in the subtype-B isolates, and with G48V and V82A \F in the non-B counterparts. This observation revealed that the non-B viruses tested had specific genotypic characteristics contrasting with the subtype-B isolates.