Comparison of motor function recovery and brain changes in intracerebral hemorrhagic and ischemic rats with similar brain damage
Comparison of motor function recovery and brain changes in intracerebral hemorrhagic and ischemic rats with similar brain damage
复制标题
DOI：
10.1097/wnr.0000000000001898
复制
发表时间：
2023-04-05
期刊：
影响因子：
1.7
通讯作者：
Iwasaki，Natsuka
中科院分区：
文献类型：
--
作者：
Tamakoshi，Keigo;Meguro，Kota;Iwasaki，Natsuka
在这项研究中，我们比较了脑出血和脑缺血的脑恢复机制，重点是突触，神经胶质细胞和多巴胺表达，这被认为是中风后神经恢复的基础。雄性Wistar大鼠分为脑出血组、脑缺血组和假手术组。脑出血组注射胶原酶溶液，脑缺血组注射内皮素-1溶液，SHAM组注射生理盐水。在手术后第7、14、21和28天，使用转棒试验评价这些大鼠的运动功能。术后第29天，使用尼氏染色分析病变体积。此外，NeuN，GFAP，酪氨酸羟化酶和PSD 95的蛋白质表达水平进行了分析，在纹状体和运动皮质。缺血组和脑出血组纹状体损伤体积无显著差异，但脑出血组运动功能恢复较缺血组快，且脑出血组运动皮质GFAP蛋白表达较缺血组高。脑出血大鼠相对于脑缺血大鼠的快速运动恢复可能与远离损伤部位的脑区星形胶质细胞的变化有关。
In this study, we compared the mechanisms of brain recovery in intracerebral hemorrhage and ischemia, focusing on synapses, glial cells, and dopamine expression, which are considered fundamental for neural recovery after stroke. Male Wistar rats were divided into intracerebral hemorrhage, ischemia, and sham surgery (SHAM) groups. The intracerebral hemorrhage group was injected with a collagenase solution, the ischemia group was injected with an endothelin-1 solution, and the SHAM group was injected with physiological saline. The motor function of these rats was evaluated using a rotarod test on days 7, 14, 21, and 28 post-surgery. On postoperative day 29, lesion volume was analyzed using Nissl staining. In addition, the protein expression levels of NeuN, GFAP, tyrosine hydroxylase, and PSD95 were analyzed in the striatum and motor cortex. There was no significant difference between the ischemia and intracerebral hemorrhage groups in terms of lesion volume in the striatum; however, the motor recovery of the intracerebral hemorrhage group occurred more rapidly than that of the ischemia group, and the intracerebral hemorrhage group exhibited higher GFAP protein expression in the motor cortex. The rapid motor recovery in intracerebral hemorrhage rats relative to that in ischemia rats may be associated with changes in astrocytes in brain regions remote from the injury site.