Brief and long maternal separations in a lupus-prone strain: Dissociation decrease corticosterone secretion from disease-related parameters
Brief and long maternal separations in a lupus-prone strain: Dissociation decrease corticosterone secretion from disease-related parameters
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DOI：
10.1016/j.bbi.2007.08.010
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发表时间：
2008-03-01
影响因子：
15.1
通讯作者：
Suchecki, Deborah
中科院分区：
文献类型：
--
作者：
Catallani, Bruna;Palma, Beatriz Duarte;Suchecki, Deborah
众所周知，新生儿手法可以改变免疫系统的活动和下丘脑-垂体-肾上腺(HPA)轴。这项研究是为了研究短暂和长期的母体分离(分别为BMS和LMS)是否干扰NZB/NZWF1雌性小鼠狼疮的发生和发展，并确定一生中皮质酮(Cort)的分泌模式是否与疾病的表达有关。母体分离在出生后第1~14天每天进行，BMS组持续15min，LMS组持续3h。分别于第9周和第10~34周龄每隔一周采血检测抗核抗体(ANA)和抗双链DNA抗体(抗dsDNA)，并测定血清皮质醇(CORT)水平。分别于出生后21、27、33、37周采集尿样。在疾病相关参数方面没有组间差异，但LMS女性表现出抗dsDNA抗体发病较晚的趋势。与非操作(NM)动物相比，BMS和LMS小鼠的皮质醇水平降低。CORT总平均浓度与ANA发病呈显著负相关，与生存期呈显著正相关，仅在NM组。新生儿操作似乎消除了这些相关性；因此，在NZB/NZWF1小鼠品系中，BMS和LMS都改变了基础皮质醇分泌以及糖皮质激素和免疫活性之间的关联。(C)2007 Elsevier Inc.保留所有权利。
Neonatal manipulations are known to alter the activity of the immune system and the hypothalamus-pituitary-adrenal (HPA) axis. This study was performed in order to examine whether brief and long maternal separations (BMS and LMS, respectively) interfere with the onset and development of murine lupus in NZB/NZWF1 females, and to determine whether the pattern of corticosterone (CORT) secretion throughout life is associated to the expression of the disease. Maternal separation was performed daily during postnatal days 1-14, lasting 15 min in the BMS group and 3 h in the LMS group. Blood was sampled from the retro-orbital plexus on the 9th week, and every other week, from 10th to 34th weeks of life, for detection of anti-nuclear antibodies (ANA) and anti-double-strand DNA (anti-dsDNA) antibodies, and for determination of CORT serum levels. Urine samples were collected on the 21st, 27th, 33rd and 37th weeks of life. There were no group differences in regard to disease-related parameters, but LMS females presented a tendency for late onset of anti-dsDNA antibodies. BMS and LMS mice exhibited reduced CORT levels compared to non-manipulated (NM) animals. There was a strong negative correlation between total mean CORT concentration and onset of ANA, and a strong positive correlation between total mean CORT concentration and life span only in the NM group. Neonatal manipulations appeared to eliminate these correlations; hence, both BMS and LMS modified basal CORT secretion and the association between glucocorticoids and immune activity in the NZB/NZWF1 mouse strain. (c) 2007 Elsevier Inc. All rights reserved.