Evolutionary and network analysis of virus sequences from infants infected with an Australian recombinant strain of human parechovirus type 3.
Evolutionary and network analysis of virus sequences from infants infected with an Australian recombinant strain of human parechovirus type 3.
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DOI：
10.1038/s41598-017-04145-2
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发表时间：
2017-06-20
影响因子：
4.6
通讯作者：
Druce J
中科院分区：
文献类型：
--
作者：
Alexandersen S;Nelson TM;Hodge J;Druce J
我们提供了近乎完整的病毒基因组序列，并对 2012 年至 2015 年期间总共 18 例澳大利亚婴儿人类副肠病毒 3 型 (HPeV3) 感染病例的潜在传播网络进行了系统发育和网络分析。总体而言，这些结果支持了我们之前的发现，即澳大利亚爆发的毒株/谱系是 2012 年 3 月至 2013 年 11 月期间发生的一次重大重组事件的结果，随后病毒进一步进化并可能发生重组。虽然来源不明的非结构编码区似乎在核苷酸和氨基酸水平上都有显着进化，但源自 HPeV3 Yamagata 2011 谱系的衣壳编码区似乎非常稳定，特别是在氨基酸水平上。系统发育和网络分析支持从 2013 年 11 月至 2014 年 3 月/4 月第一个澳大利亚重组病毒序列到 2015 年爆发的时间演化。 2015年的疫情样本分为两个独立的簇，在2014年3月/4月至2015年9月之间可能有共同的祖先，每个簇在2015年9月至11月/12月期间进一步演变。
We present the near complete virus genome sequences with phylogenetic and network analyses of potential transmission networks of a total of 18 Australian cases of human parechovirus type 3 (HPeV3) infection in infants in the period from 2012–2015. Overall the results support our previous finding that the Australian outbreak strain/lineage is a result of a major recombination event that took place between March 2012 and November 2013 followed by further virus evolution and possibly recombination. While the nonstructural coding region of unknown provenance appears to evolve significantly both at the nucleotide and amino acid level, the capsid encoding region derived from the Yamagata 2011 lineage of HPeV3 appears to be very stable, particularly at the amino acid level. The phylogenetic and network analyses performed support a temporal evolution from the first Australian recombinant virus sequence from November 2013 to March/April 2014, onto the 2015 outbreak. The 2015 outbreak samples fall into two separate clusters with a possible common ancestor between March/April 2014 and September 2015, with each cluster further evolving in the period from September to November/December 2015.