Simultaneous determination of nicotine and cotinine in human plasma by nitrogen detection gas-liquid chromatography.
Simultaneous determination of nicotine and cotinine in human plasma by nitrogen detection gas-liquid chromatography.
复制标题
氮检测气液色谱法同时测定人血浆中尼古丁和可替宁的含量
作者：
Kogan Mj;K. Verebey;J. H. Jaffee;S. Mulé
采用气液色谱法结合氮选择性检测法同时定量测定人血浆中尼古丁及其主要代谢产物可替宁的浓度。将尼古丁、可替宁和添加的内标物氯胺酮在碱性pH值下从血浆中提取至二氯甲烷中，反提取至酸中，然后再提取至二氯甲烷中。在3%SE-30填充玻璃柱上进行分析，柱温编程为150 - 200 ℃。检测器响应在2 - 50 ng/mL尼古丁和50 - 500 ng/mL可替宁范围内呈线性。该方法在150份来自习惯性吸烟者的血浆样品上进行了验证。尼古丁和可替宁的平均水平分别为19.5和219 ng/mL。水平的平均值和范围均与先前报告的尼古丁和可替宁血浆水平一致。
Human plasma levels of nicotine and its principal metabolite, cotinine, were simultaneously quantitated by gas-liquid chromatography combined with nitrogen selective detection. Nicotine, cotinine, and the added internal standard ketamine are extracted from plasma at basic pH into methylene chloride, back-extracted into acid, and then re-extracted into methylene chloride. Analysis is carried out on a packed glass column of 3% SE-30 while column temperature is programmed from 150 to 200 degrees C. Detector response is linea over the range of 2 to 50 ng/mL nicotine and 50 to 500 ng/mL cotinine. The method was validated on 150 plasma samples obtained from habitual smokers. Mean levels of 19.5 and 219 ng/mL were found for nicotine and cotinine, respectively. Both the mean and the range of the levels were in agreement with previously reported plasma levels for nicotine and cotinine.