Outer membrane mutants of Salmonella typhimurium LT2 have lipopolysaccharide-dependent resistance to the bactericidal activity of anaerobic human neutrophils.
Outer membrane mutants of Salmonella typhimurium LT2 have lipopolysaccharide-dependent resistance to the bactericidal activity of anaerobic human neutrophils.
复制标题
鼠伤寒沙门氏菌 LT2 的外膜突变体对人类厌氧中性粒细胞的杀菌活性具有脂多糖依赖性抵抗力。
DOI：
10.1128/iai.36.3.1086-1095.1982
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Spitznagel,JK
中科院分区：
文献类型：
--
作者：
Okamura,N;Spitznagel,JK
中性粒细胞多形性粒细胞（PMN）在厌氧和有氧条件下吞噬细菌的能力提供了机会，以比较与鼠伤寒沙门氏菌LT 2及其脂多糖突变体（外膜突变体）挑战的人PMN中的氧非依赖性和氧依赖性抗菌机制的杀菌活性。厌氧人中性粒细胞的挑战，无论是调理LT 2或血清处理的酵母聚糖未能产生可检测的超氧阴离子（O2-）或减少氮蓝四唑，虽然好氧中性粒细胞容易产生O2-在响应这样的挑战。厌氧中性粒细胞杀死这些细菌在一个有序的方式，似乎是依赖于他们的脂多糖化学型。随着突变体脂多糖的碳水化合物含量的降低，细菌对不依赖于氧的杀菌活性的抗性降低。结果类似于观察到的有序耐氧非依赖性杀伤与LT 2及其突变体在中性粒细胞无系统与中性粒细胞颗粒蛋白。对杀灭动力学的研究表明，与需氧条件相比，厌氧条件下的杀灭速度较慢。调理作用增加了吞噬率，但调理作用和吞噬率等因素似乎并不影响白细胞内杀菌能力，因为无论使用哪种血清（正常血清、C6缺乏血清、C8缺乏血清或根本没有血清），摄入后细菌存活的比例都是相似的。结果是一致的积极和大量参与的氧非依赖性系统中的嗜中性粒细胞的抗菌作用。
The capacity of neutrophil polymorphonuclear granulocytes (PMNs) to phagocytize bacteria under anaerobic as well as aerobic conditions afforded the opportunity to compare the bactericidal activities of oxygen-independent and oxygen-dependent antimicrobial mechanisms in human PMNs challenged with Salmonella typhimurium LT2 and its lipopolysaccharide mutants (outer membrane mutants). Anaerobic human PMNs challenged with either opsonized LT2 or serum-treated zymosan failed to produce detectable superoxide anion (O2-) or to reduce nitroblue tetrazolium, although aerobic PMNs readily produced O2- in response to such challenge. Anaerobic PMNs killed these bacteria in an ordered fashion that appeared to be dependent on their lipopolysaccharide chemotype. As the carbohydrate content of the mutant lipopolysaccharide decreased, the bacteria became less resistant to the oxygen-independent bactericidal activity. The results resembled the ordered resistance to oxygen-independent killing observed with LT2 and its mutants in PMN-free systems with PMN granule proteins. Studies on the kinetics of killing showed these to be less rapid in anaerobic as compared with aerobic conditions. Opsonization increased the rate of phagocytosis, but such factors as opsonization and the rate of phagocytosis did not appear to affect intraleukocytic bactericidal capacity in that the resultant proportion of bacteria remaining viable after ingestion was similar regardless of which serum was used (normal serum, C6-deficient serum, C8-deficient serum, or no serum at all). The results are consistent with an active and substantial participation by oxygen-independent systems in the antimicrobial effects of neutrophils.