Screening for disease resistance in barley cultivars against Bipolaris sorokiniana using callus culture method.
Screening for disease resistance in barley cultivars against Bipolaris sorokiniana using callus culture method.
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发表时间：
2008-04
影响因子：
0.6
通讯作者：
R. Chand;D. Sen;K. Prasad;A. Singh;B. Bashyal;L. Prasad;A. Joshi
中科院分区：
文献类型：
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作者：
R. Chand;D. Sen;K. Prasad;A. Singh;B. Bashyal;L. Prasad;A. Joshi
在直立大麦植株上以及在选择的大麦愈伤组织系中评估响应于小离蠕孢真菌毒素的抗性大麦基因型的筛选。对于测试的站立品系，与坏死品系相比，那些响应于毒素渗透而表现出失绿的品系显示出显著较慢的疾病进展。此外，在MS培养基中补充不同浓度的粗毒素的感病品种的愈伤组织中的坏死显着高于研究的黄化系的愈伤组织。在体外和田间条件下，类似的宿主对毒素的反应开辟了利用愈伤组织培养筛选大麦品种对斑点斑点病的抗性的可能性，而不是经典的筛选方法，以提高准确性并节省时间和空间。
Screening for resistant barley genotypes in response to fungal toxin of Bipolaris sorokiniana was assessed on standing barley plants as well as in selected callus lines of the same. For the standing lines tested, those manifesting chlorosis in response to toxin infiltration showed a significantly slower disease progress as compared to the necrotic lines. Also, necrosis in the callus tissues of the susceptible cultivar in MS medium supplemented with different concentrations of the crude toxin was significantly higher than in the callus tissues of the chlorotic lines studied. Similar host response to the toxin in in vitro and field situations open up the possibility of screening barley cultivars for resistance to spot blotch using callus culture as against classical methods of screening in order to increase accuracy and save time and space.