Adsorption of Follicular Dendritic Cell-secreted Protein (FDC-SP) onto Mineral Deposits
Adsorption of Follicular Dendritic Cell-secreted Protein (FDC-SP) onto Mineral Deposits
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滤泡树突状细胞分泌蛋白 (FDC-SP) 吸附到矿物质沉积物上
DOI：
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发表时间：
2008
影响因子：
4.8
通讯作者：
J. Kimura
中科院分区：
文献类型：
--
作者：
T. Shinomura;Sayaka Nakamura;Kazuo Ito;S. Shirasawa;M. Höök;J. Kimura
滤泡树突状细胞分泌蛋白（FDC-SP）是一种小的分泌蛋白，与唾液中被认为在唾液中的钙保留中起作用的蛋白-唾液酸甘油酶具有结构相似性。相反，FDC-SP被认为在与生发中心相关的免疫系统中发挥作用。我们在这里报告的非常具体的表达FDC-SP在交界上皮在龈沟。该区域对于宿主防御病原体和牙周保护非常重要。为了能够更好地了解FDC-SP的功能，我们开发了一种新的基因表达系统，该系统利用基因捕获和位点特异性基因整合将蛋白质引入哺乳动物细胞培养系统。使用该系统，我们能够在成骨祖细胞系中表达FDC-SP作为具有长期稳定性的绿色荧光蛋白的融合蛋白，然后我们使用该系统发现融合蛋白特异性吸附到矿物沉积物和外源添加到培养物中的羟基磷灰石颗粒的表面上。FDC-SP的吸附依赖于其结构的完整性。这些结果表明，FDC-SP可能发挥了重要作用，吸附到牙骨质和牙槽骨表面邻近牙周膜和牙齿表面的龈沟。
Follicular dendritic cell-secreted protein (FDC-SP) is a small secretory protein having structural similarities to statherin, a protein in saliva thought to play a role in calcium retention in saliva. In contrast, FDC-SP is thought to play a role in the immune system associated with germinal centers. We report here the very specific expression of FDC-SP in junctional epithelium at the gingival crevice. This region is very important for the host defense against pathogens and for periodontal protection. To be able to better understand the function of FDC-SP, we developed a novel gene expression system that exploited gene trapping and site-specific gene integration to introduce the protein into a mammalian cell culture system. Using this system we were able to express FDC-SP as a fusion protein with green fluorescent protein in an osteogenic progenitor cell line with long term stability, which we then used to find that the fusion protein specifically adsorbs onto mineral deposits and the surface of hydroxyapatite particles exogenously added to the culture. This adsorption was highly dependent on the structural integrity of FDC-SP. These results suggest that FDC-SP may play an important role, adsorbing onto the surface of cementum and alveolar bone adjacent to periodontal ligament and onto tooth surface at the gingival crevice.
影响因子：
4.4
作者：
Dodds, MWJ;Johnson, DA;Yeh, CK
通讯作者：
Yeh, CK