Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection
Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection
复制标题
具有发光二极管诱导荧光检测功能的集成直接接触光纤微芯片的开发
DOI：
10.1016/j.chroma.2007.08.087
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发表时间：
2007-11-02
影响因子：
4.1
通讯作者：
Chen, Xing
中科院分区：
文献类型：
--
作者：
Liu, Changchun;Cui, Dafu;Chen, Xing
本文采用薄铸法制备了一种集成了一根直接接触光纤的聚二甲基硅氧烷（PDMS）夹层微芯片。该新型集成PDMS夹层微芯片包括顶部玻璃板、带有微流控网络和光纤的PDMS膜复制品、平板PDMS膜和底部玻璃板。由于该集成微芯片的激发光纤尖端与分离微通道完全接触，既增加了激发光强度，实现了高灵敏度，又减小了激发光束直径，获得了高分辨率。此外，我们发现这种刚性PDMS夹层微芯片结构有效地防止了刚性光纤对PDMS微通道的畸变，为微芯片的操作提供了极大的便利。采用蓝色发光二极管（LED）作为激发源，利用光纤将激发光耦合到其直接接触的微通道中进行荧光检测。通过分离荧光素钠（SF）和异硫氰酸荧光素异构体I （FITC）的混合物，证明了该集成PDMS三明治微芯片的性能，并且在光纤尖端与分离微通道之间距离为100 μ m时，显示出比传统集成光纤PDMS微芯片更高的灵敏度和分辨率。此外，还通过分离fitc标记的氨基酸混合物来检查该集成微芯片与led诱导荧光检测的可重复性。(C) 2007 Elsevier B.V.版权所有
In this paper, one poly(dimethylsiloxane) (PDMS) sandwich microchip integrated with one direct-contacting optical fiber was fabricated by using a thin-casting method. This novel integrated PDMS sandwich microchip included top glass plate, PDMS membrane replica with microfluidic networks and optical fiber, flat PDMS membrane and bottom glass plate. As the tip of excitation optical fiber completely contacted with the separation microchannel in this integrated microchip, it not only increased the excitation light intensity to achieve the high sensitivity, but also reduced the diameter of excitation beam to obtain high resolution. In addition, we found that this rigid PDMS sandwich microchip structure effectively prevented PDMS microchannel distortion from rigid optical fiber, and provided a substantial convenience for microchips manipulating. A blue light-emitting diode (LED) was applied as excitation source by using optical fiber to couple excitation light into its direct-contacting microchannel for fluorescence detection. The performances of this integrated PDMS sandwich microchip was demonstrated by separating the mixture of sodium fluorescein (SF) and fluorescein isothiocyanate isomer I (FITC), and showed a higher sensitive and resolution than those obtained from the conventional integrated optical-fiber PDMS microchip with a 100-mu m distance between fiber tip and separation microchannel. Additionally, the reproducibility of this integrated microchip with LED-induced fluorescence detection was also examined by separation of a mixture of FITC-labeled amino acids. (C) 2007 Elsevier B.V. All rights reserved.