The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants
The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants
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DOI：
10.1007/s12275-017-7362-y
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发表时间：
2017-10-01
影响因子：
3
通讯作者：
Han, Seung Hyun
中科院分区：
文献类型：
--
作者：
Ahn, Ki Bum;Kim, A. Reum;Han, Seung Hyun
变形链球菌是人类龋齿的主要病原体，其在人类口腔中的硬组织（例如牙齿和牙本质表面）上形成生物膜。人β-防御素-3（HBD 3）是一种由45个氨基酸组成的天然抗菌肽，具有广谱抗菌活性。由HBD 3的C-末端15个氨基酸组成的合成肽（HBD 3-C15）最近被证明足以具有其抗微生物活性。因此，这种肽的临床应用已引起关注。在这项研究中，我们研究了HBD 3-C15是否抑制代表性致龋病原体变形链球菌的生长及其生物膜形成。HBD 3-C15抑制细菌生长，表现出杀菌活性，并以剂量依赖性方式减弱细菌生物膜形成。HBD 3-C15增强了氢氧化钙（CH）和氯己定二葡萄糖酸盐（CHX）的杀菌和抗生物膜活性，这是牙科诊所中使用的代表性消毒剂，对S。变异人HBD 3-C15还能抑制S.变形菌和其它嗜牙本质菌，如粪肠球菌和戈登链球菌，它们与龋齿和牙髓感染有关。在HBD 3-C15单独给药和HBD 3-C15与CH或CHX联合给药时观察到这些效应。因此，我们认为HBD 3-C15是一种潜在的替代或添加消毒剂，可用于治疗口腔感染性疾病，包括龋齿和牙髓感染。
Streptococcus mutans is a major etiologic agent of human dental caries that forms biofilms on hard tissues in the human oral cavity, such as tooth and dentinal surfaces. Human (3-defensin-3 (HBD3) is a 45-amino-acid natural antimicrobial peptide that has broad spectrum antimicrobial activity against bacteria and fungi. A synthetic peptide consisting of the C-terminal 15 amino acids of HBD3 (HBD3-C15) was recently shown to be sufficient for its antimicrobial activity. Thus, clinical applications of this peptide have garnered attention. In this study, we investigated whether HBD3-C15 inhibits the growth of the representative cariogenic pathogen Streptococcus mutans and its biofilm formation. HBD3-C15 inhibited bacterial growth, exhibited bactericidal activity, and attenuated bacterial biofilm formation in a dose-dependent manner. HBD3-C15 potentiated the bactericidal and anti-biofilm activity of calcium hydroxide (CH) and chlorhexidine digluconate (CHX), which are representative disinfectants used in dental clinics, against S. mutans. Moreover, HBD3-C15 showed antimicrobial activity by inhibiting biofilm formation by S. mutans and other dentinophilic bacteria such as Enterococcus faecalis and Streptococcus gordonii, which are associated with dental caries and endodontic infection, on human dentin slices. These effects were observed for HBD3-C15 alone and for HBD3-C15 in combination with CH or CHX. Therefore, we suggest that HBD3-C15 is a potential alternative or additive disinfectant that can be used for the treatment of oral infectious diseases, including dental caries and endodontic infections.