Ice-Nucleating Active Bacteria Decrease the Cold-Hardiness of Stored Grain Insects
Ice-Nucleating Active Bacteria Decrease the Cold-Hardiness of Stored Grain Insects
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冰核活性细菌降低储存谷物昆虫的耐寒性
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
Evelyn C. Davidson
中科院分区：
文献类型：
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作者：
R. Lee;J. Strong;Marcia R. Lee;Evelyn C. Davidson
这份报告提供了进一步的证据表明，冷冻干燥，浓缩形式的假单胞菌，冰核活性细菌，降低了耐寒性的粮食害虫。将冰核细菌应用于含有害虫的小麦或玉米降低了昆虫的过冷却能力：在用100 ppm的P. dichloringae处理后，五种昆虫的平均过冷却点从4.7 ° C增加到11.9°C，高于未经处理的对照。用P. dichloringae处理也降低了昆虫在零下温度下暴露24小时的生存能力。在8种储粮害虫中观察到耐冷性下降：印度谷螟幼虫，Plodia interpullctella（Hubner）;赤拟谷盗成虫，赤拟谷盗（Tribolium castaneum（Herbst））;扁谷盗成虫，Cryptolestes pusillus（Schon herr）;锈谷盗成虫，Cryptolestes ferrugineus（Stephens）;赤霉病（Gibbium psylloides（Czenpinski））;小谷盗成虫，谷蠹（Rhyzopertha dominica（F.）;黄粉虫幼虫，黄粉虫;和谷象成虫Sitophilus granarius granarius（L.）.本研究结果为冰核活性细菌作为生物杀虫剂降低越冬昆虫的低温耐受性提供了进一步的支持。该方法可能特别适合于控制限制区域如粮仓中的各种昆虫害虫。
This report provides further evidence that a freeze-dried, concentrated form of Pseudomonas syringae , an ice-nucleating active bacteria, reduces the cold tolerance of stored grain insect pests. Application of ice-nucleating bacteria to wheat or corn that contained insect pests decreased the insects’ supercooling capacity: after treatment with 100 ppm of P. syringae the mean supercooling points of five insect species increased from 4.7 to 11.9°C above untreated controls. Treatment with P. syringae also decreased the capacity of insects to survive a 24-h exposure to subzero temperatures. Decreases in cold tolerance were observed in eight species of stored grain pests: Indianmeal moth larvae, Plodia interpullctella (Hubner); red flour beetle adults, Tribolium castaneum (Herbst); flat grain beetle adults, Cryptolestes pusillus (Schon herr); rusty grain beetle adults, Cryptolestes ferrugineus (Stephens); Gibbium psylloides (Czenpinski); lesser grain borer adults, Rhyzopertha dominica (F.); yellow meal worm larvae, Tenebrio molitor (L.); and granary weevil adults, Sitophilus granarius granarius (L.). Results of this study provide further support for the use of ice-nucleating active bacteria as biological insecticides to kill overwintering insects by decreasing their low temperature tolerance. The approach may be particularly appropriate for the control of a variety of insect pests in restricted areas such as grain bins.