Overproduced p73α activates a minimal promoter through a mechanism independent of its transcriptional activity
Overproduced p73α activates a minimal promoter through a mechanism independent of its transcriptional activity
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过量产生的 p73α 通过与其转录活性无关的机制激活最小启动子
DOI：
10.1016/s0014-5793(01)03141-6
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发表时间：
2001
期刊：
影响因子：
3.5
通讯作者：
K. Shimotohno
中科院分区：
文献类型：
--
作者：
S. Takagi;Y. Ueda;M. Hijikata;K. Shimotohno
p73是与肿瘤抑制因子p53相关的蛋白质的基因，其编码几种变体，这些变体由于选择性剪接而具有不同的羧基末端结构。我们和其他人表明，这些剪接变体对具有p53结合共有序列（p53 BCS）的启动子具有不同的转录作用。在这里，我们发现，当瞬时过表达时，p73α而不是p73β激活了几个不含p53 BCS的最小启动子，而p73γ和p73 β激活它们的程度远低于p73α，而p53抑制了不含p53 BCS的启动子。此外，RNase保护和RNA转染试验的结果表明，这种激活发生在转录水平。对p73α的缺失分析表明，p73 α的反式激活结构域不参与这种活性，而p73α的C端区域是这种变异体的一种特殊结构，这表明这种现象的发生不依赖于p73α的反式激活活性，p73α的C端延伸可能影响转录的基础水平。
p73, the gene for a protein related to the tumor suppressor p53, encodes several variants which bear distinct carboxy-terminal structures as a result of alternative splicing. We and others showed that these splicing variants have different transcriptional effects on promoters with a p53-binding consensus sequence (p53BCS). Here we show that when transiently overexpressed, p73α but not p73β activated several minimal promoters without the p53BCS, while p73γ and p73ϵ activated them to a much lesser extent than p73α, and p53 suppressed the promoters without p53BCS as reported previously. Moreover, the results of RNase protection and RNA transfection assays suggested that this activation occurred at the transcriptional level. Deletion analysis of p73α revealed that the transactivation domain of p73 was not involved in this activity and the C-terminal region of p73α which is a specific structure of this variant was essential, suggesting that this phenomenon occurs independent of the transactivation activity of p73α and that the C-terminal extension of p73α may affect the basal level of transcription.
p73 和 p53 对细胞和病毒启动子的差异调节。
影响因子：
5.2
作者：
Deb,D;Lanyi,A;Scian,M;Keiger,J;Brown,DR;LeRoith,D;Deb,SP;Deb,S
通讯作者：
Deb,S
影响因子：
10.5
作者：
Murphy, M;Ahn, J;George, DL
通讯作者：
George, DL