Isolation of halotolerant Lactococcus lactis subsp lactis from intestinal tract of coastal fish
Isolation of halotolerant Lactococcus lactis subsp lactis from intestinal tract of coastal fish
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DOI：
10.1016/j.ijfoodmicro.2007.11.031
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发表时间：
2008-01-15
影响因子：
5.4
通讯作者：
Sugita, Haruo
中科院分区：
文献类型：
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作者：
Itoi, Shiro;Abe, Takeshi;Sugita, Haruo
我们从日本静冈下田捕获的东方鲀（Takifugu niphobles）的肠道中分离乳酸菌。使用表型测试如革兰氏染色、细胞形态、过氧化氢酶、氧化酶和葡萄糖发酵进行额外的筛选。随后，对表型试验筛选的227株乳酸乳球菌进行种特异性PCR，得到4株阳性菌株。3个分离株的16SrRNA基因序列与L. lactis subsp.乳酸菌（DNA数据库登录号M58837），而一个分离物的序列与肠膜明串珠菌（AB 023246）的序列相同。用API 50 CH糖发酵和耐盐表型鉴定标准对这些菌株进行了鉴定，并与L. lactis subsp.来自奶酪发酵剂培养物的乳酸。这些菌株的碳水化合物发酵谱具有L. lactis subsp. lactis菌株的耐盐性，而这些菌株的耐盐性高于L. lactis subsp.乳酸菌：新的L。乳酸菌菌株在含200%海水或6%氯化钠的MRS琼脂平板上表现出较高的耐盐性。本文首次报道了耐盐L. lactis subsp.来自海洋环境的乳酸菌。(c)2007 Elsevier B.V保留所有权利。
We isolated lactic acid bacteria from the intestinal tract of the pufferfish Takifugu niphobles caught in Shimoda, Shizuoka, Japan by using MRS broth prepared with 50% seawater. Additional screening was carried out using phenotypic tests such as Gram staining, cell morphology, catalase, oxidase and fermentation of glucose. Subsequently 227 isolates screened by the phenotypic tests were subjected to species- specific PCR for Lactococcus lactis, resulting in four positive isolates. The 16S rRNA gene sequences from three isolates were highly similar to that of L. lactis subsp. lactis (DNA database accession number M58837), while that of one isolate was identical to that of Leuconostoc mesenteroides (AB023246). These isolates were characterized by API 50 CH for carbohydrate fermentation and other phenotypic criteria for salt tolerance, and the characteristics were compared with those of L. lactis subsp. lactis from a cheese starter culture. The carbohydrate fermentation profiles of these isolates were characteristic of L. lactis subsp. lactis strains, whereas the tolerance of these isolates to salt was higher than that of L. lactis subsp. lactis from the cheese starter culture: the new L. lactis isolates showed high salt tolerance in MRS-agar plates containing 200% seawater or 6% sodium chloride. This is the first report of the isolation of halotolerant strains of L. lactis subsp. lactis from a marine environment. (c) 2007 Elsevier B.V All rights reserved.