On the isobaric space of 25-hydroxyvitamin D in human serum: potential for interferences in liquid chromatography/tandem mass spectrometry, systematic errors and accuracy issues.
On the isobaric space of 25-hydroxyvitamin D in human serum: potential for interferences in liquid chromatography/tandem mass spectrometry, systematic errors and accuracy issues.
复制标题
人血清中 25-羟基维生素 D 的同量异位空间：液相色谱/串联质谱法的潜在干扰、系统误差和准确性问题
DOI：
10.1002/rcm.7075
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Volmer DA
中科院分区：
文献类型：
--
作者：
Geib T;Schorr P;Meier F;Volmer DA
原理当采用低分辨力液相色谱/串联质谱（LC/MS/MS）仪器和非特异性MS/MS产物离子进行分析时，人血清中的等压干扰可能会影响25-羟基维生素D [25（OH）D]的测定浓度水平。在这项研究中，我们提供了一个详细的表征，这些干扰和技术解决方案，以减少相关的系统errors.METHODSDetailed电喷雾电离傅立叶变换离子回旋共振（FTICR）高分辨率质谱（HRMS）实验被用来表征共提取的同量异位素成分的25（OH）D从人血清。差示离子迁移谱（DMS）作为气相离子过滤器，在三重四极杆质谱仪上实现了同量异位素的分离。在不同样品制备方法下，人血清提取物中存在多种同量异位素化合物。其中一些同量异位素有可能增加低分辨率MS仪器上25（OH）D的峰面积。一个主要的等压组分被确定为季戊四醇油酸酯，一种技术润滑剂，这可能是一个人工制品的分析仪器。当在低分辨率三重四极杆质谱仪上实施时，DMS能够在MS/MS之前去除其中几个同量异位素。结论在此概念验证研究中显示，DMS-MS具有显著降低系统误差的潜力，从而提高使用LC/MS/MS测量维生素D的准确性。
RATIONALEIsobaric interferences in human serum can potentially influence the measured concentration levels of 25‐hydroxyvitamin D [25(OH)D], when low resolving power liquid chromatography/tandem mass spectrometry (LC/MS/MS) instruments and non‐specific MS/MS product ions are employed for analysis. In this study, we provide a detailed characterization of these interferences and a technical solution to reduce the associated systematic errors.METHODSDetailed electrospray ionization Fourier transform ion cyclotron resonance (FTICR) high‐resolution mass spectrometry (HRMS) experiments were used to characterize co‐extracted isobaric components of 25(OH)D from human serum. Differential ion mobility spectrometry (DMS), as a gas‐phase ion filter, was implemented on a triple quadrupole mass spectrometer for separation of the isobars.RESULTSHRMS revealed the presence of multiple isobaric compounds in extracts of human serum for different sample preparation methods. Several of these isobars had the potential to increase the peak areas measured for 25(OH)D on low‐resolution MS instruments. A major isobaric component was identified as pentaerythritol oleate, a technical lubricant, which was probably an artifact from the analytical instrumentation. DMS was able to remove several of these isobars prior to MS/MS, when implemented on the low‐resolution triple quadrupole mass spectrometer.CONCLUSIONSIt was shown in this proof‐of‐concept study that DMS‐MS has the potential to significantly decrease systematic errors, and thus improve accuracy of vitamin D measurements using LC/MS/MS. Copyright © 2014 John Wiley & Sons, Ltd.
DOI：
--
发表时间：
1980-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Kishimoto;Y. Takai;T. Mori;U. Kikkawa;Y. Nishizuka
通讯作者：
A. Kishimoto;Y. Takai;T. Mori;U. Kikkawa;Y. Nishizuka
微分离子淌度谱中气相离子/中性相互作用的描述：使用校准运行进行 CV 预测
影响因子：
3.2
作者：
Auerbach D;Aspenleiter J;Volmer DA
通讯作者：
Volmer DA