OUTCOME MODEL OF ASPHYXIAL CARDIAC-ARREST IN RATS
OUTCOME MODEL OF ASPHYXIAL CARDIAC-ARREST IN RATS
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DOI：
10.1038/jcbfm.1995.129
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发表时间：
1995-11-01
影响因子：
6.3
通讯作者：
NEUMAR, R
中科院分区：
文献类型：
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作者：
KATZ, L;EBMEYER, U;NEUMAR, R
为了量化脑损伤，建立了一种大鼠窒息心脏骤停的结局模型。22只大鼠被随机分为三组。对照组I：正常、清醒、无窒息(n=6)。假手术组(Sham II)6只，麻醉后手术，无窒息。停跳组(Ⅲ组，n=10)：轻度麻醉，窒息8min，停跳后3~4min停止循环，心跳停止(无血流)4~5min，10只经标准体外心肺复苏恢复自主循环。9只大鼠存活1h，观察至72h，1只大鼠在拔管前死亡。所有9名幸存者在72小时内都是清醒的，神经功能缺失评分(0%=最好；100%=最差)为7+/-6%(2-16%)。对五个冠状水平的所有脑区进行了缺血神经元检查。对五个区域的缺血神经元的患病率进行绝对评分。III组(n=9)的平均脑组织病理损伤评分为2.1(P<；0.05 vs I组或II组)，建立了可复制的大鼠心脏骤停模型。它为研究短暂性全脑缺氧缺血性脑损伤所致神经元死亡的病理生理学机制提供了工具。
An outcome model with asphyxial cardiac arrest in rats has been developed for quantifying brain damage, Twenty-two rats were randomized into three groups. Control group I was normal, was conscious, and had no asphyxia (n = 6). Sham group II had anesthesia and surgery but no asphyxia (n = 6). All 12 rats in groups I and II survived to 72 h and were functionally and histologically normal, Arrest group III (the model; n = 10) had light anesthesia and apneic asphyxia of 8 min, which led to cessation of circulation at 3-4 min of apnea, resulting in cardiac arrest (no flow) of 4-5 min, All 10 rats had spontaneous circulation restored by standard external cardiopulmonary resuscitation. Nine rats survived controlled ventilation for I h and observation to 72 h, while one rat died before extubation. All nine survivors were conscious at 72 h, with neurologic deficit scores (0% = best; 100% = worst) of 7 +/- 6% (2-16%). All brain regions at five coronal levels were examined for ischemic neurons. The prevalence of ischemic neurons in five regions was categorically scored. The average total brain histopathologic damage score in group III (n = 9) was 2.1 (p < 0.05 vs, group I or II), A reproducible outcome model of cardiac arrest in rats was documented. It provides a tool for investigating pathophysiological mechanisms of neuronal death caused by a transient global hypoxic-ischemic brain insult.