Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa
Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa
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DOI：
10.1016/j.mimet.2011.02.013
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发表时间：
2011-06-01
影响因子：
2.2
通讯作者：
Bie, Xiaomei
中科院分区：
文献类型：
--
作者：
Deng, Yang;Lu, Zhaoxin;Bie, Xiaomei
多粘类芽孢杆菌JSA-9是一株在体外具有抗菌和抗真菌活性的土壤菌株，已发现产生两种类型的抗菌物质。用乙酸乙酯从JSA-9发酵液中提取这两个化合物，并用高效液相色谱分离纯化。通过液-质联用分析，鉴定出两种拮抗物质中的一种为邻苯二甲酸二丁酯。另一种是分子质量为883、897、911、947和961Da的相关多肽的混合物，它们的结构最有可能是一个环状脱脂肽，其中一个不寻常的15-胍基-3-羟基十五烷酸部分结合在一个自由氨基上。因此，这些多肽是环多肽的Li-F基团的成员。(C)2011爱思唯尔B.V.保留所有权利。
Paenibacillus polymyxa strain JSa-9, a soil isolate that displayed antibacterial and antifungal activities in vitro, had been found to produce two types of antimicrobial substances. The two compounds were extracted from the fermentation broth of JSa-9 using ethyl acetate and subsequently purified by high performance liquid chromatography. By means of liquid chromatography-mass spectrometry and tandem mass spectrometry analysis, one of two antagonistic compounds was determined as di-n-butyl phthalate. And another was characterized as a mixture of related peptides of molecular masses of 883, 897, 911, 947, and 961 Da, with the most likely structure of them determined to be a cyclic depsipeptide with an unusual 15-guanidino-3-hydroxypentadecanoic acid moiety bound to a free amino group. These peptides were therefore members of the LI-F group of cyclic depsipeptides. (C) 2011 Elsevier B.V. All rights reserved.