Radiosensitization effect of folate-conjugated gold nanoparticles on HeLa cancer cells under orthovoltage superficial radiotherapy techniques
Radiosensitization effect of folate-conjugated gold nanoparticles on HeLa cancer cells under orthovoltage superficial radiotherapy techniques
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DOI：
10.1088/0031-9155/59/9/2249
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发表时间：
2014-05-07
影响因子：
3.5
通讯作者：
Soleimani, Masoud
中科院分区：
文献类型：
--
作者：
Khoshgard, Karim;Hashemi, Bijan;Soleimani, Masoud
由于金纳米粒子（GNPs）的高原子序数，它们被称为新的放射增敏剂，通过增加肿瘤细胞吸收的剂量来提高浅表放射治疗技术的效率，从而可以选择性地积累。本研究的目的是比较各种常见的低能量水平的正压x射线和巨压伽马射线（Co-60）对在共轭叶酸和非共轭（聚乙二醇化）GNPs存在下提高HeLa癌细胞治疗效率的影响。为了实现这一目标，合成了平均直径为52 nm的GNPs并将其偶联到叶酸分子上。还合成了平均直径为47 nm的聚乙二醇化GNPs，并用作非共轭叶酸GNPs。合成folate-conjugated细胞毒性试验和聚乙二醇国民生产总值进行了使用不同级别的纳米颗粒浓度与海拉细胞孵化24 h。共轭和聚乙二醇的机体影响国民生产总值在细胞的浓度为50μM比较使用MTT以及单独使用化验后暴露他们2 Gy电离辐射产生的一个中电压x光机的四种不同千伏峰值和伽马射线Co-60单位。叶酸偶联和聚乙二醇化GNPs的平均剂量增强因子（DEF）分别为1.64 +/- 0.05和1.35 +/- 0.05，不同辐照组间存在显著差异。在180 kVp的x射线束下，两种GNPs的DEF都达到了最大值。叶酸偶联GNPs可以显著增强正电压x射线能量（特别是180 kVp）对表达叶酸受体的癌细胞（如HeLa）的细胞杀伤潜力。
Due to the high atomic number of gold nanoparticles (GNPs), they are known as new radiosensitizer agents for enhancing the efficiency of superficial radiotherapy techniques by increasing the dose absorbed in tumor cells wherein they can be accumulated selectively. The aim of this study was to compare the effect of various common low energy levels of orthovoltage x-rays and megavoltage gamma-rays (Co-60) on enhancing the therapeutic efficiency of HeLa cancer cells in the presence of conjugated folate and non-conjugated (pegylated) GNPs. To achieve this, GNPs with an average diameter of 52 nm were synthesized and conjugated to folic acid molecules. Pegylated GNPs with an average diameter of 47 nm were also synthesized and used as non-conjugated folate GNPs. Cytotoxicity assay of the synthesized folate-conjugated and pegylated GNPs was performed using different levels of nanoparticle concentration incubated with HeLa cells for 24 h. The radiosensitizing effect of both the conjugated and pegylated GNPs on the cells at a concentration of 50 mu M was compared using MTT as well as clonogenic assays after exposing them to 2 Gy ionizing radiation produced by an orthovoltage x-ray machine at four different kVps and gamma-rays of a Co-60 unit. Significant differences were noted among various irradiated groups with and without the folate conjugation, with an average dose enhancement factor (DEF) of 1.64 +/- 0.05 and 1.35 +/- 0.05 for the folate-conjugated and pegylated GNPs, respectively. The maximum DEF was obtained with the 180 kVp x-ray beam for both of the GNPs. Folate-conjugated GNPs can significantly enhance the cell killing potential of orthovoltage x-ray energies (especially at 180 kVp) in folate receptor-expressing cancer cells, such as HeLa, in superficial radiotherapy techniques.