Age-related and infection intensity-related shifts in antibody recognition of defined protein antigens in a schistosome-exposed population
Age-related and infection intensity-related shifts in antibody recognition of defined protein antigens in a schistosome-exposed population
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DOI：
10.1086/589511
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发表时间：
2008-07-15
影响因子：
6.4
通讯作者：
Maizels, Rick M.
中科院分区：
文献类型：
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作者：
Mutapi, Francisca;Burchmore, Richard;Maizels, Rick M.
背景资料。这项研究比较了5-18岁的津巴布韦血吸虫感染者的血清抗体对血吸虫成虫抗原的识别模式。根据年龄和感染强度将人群分成9组，其中血清样本被汇集在一起，用于通过二维Western blotting筛选蛋白质识别。用电喷雾电离串联质谱仪鉴定识别的蛋白质。共有71个抗原被~gt；=1个血清池识别。这种识别能力随寄主年龄和侵染强度的不同而明显不同，具有一定的异构体特异性反应。可识别的抗原谱系随着年龄的增长而增加，在年龄最大的参与者中达到顶峰，他们没有感染强度或轻度至中度感染强度。抗原识别的强度也随着年龄的增长而增加，在感染强度最大的年龄组达到峰值。对特定血吸虫抗原的识别，无论是识别抗原的多样性还是识别抗原的强度，都随着感染时间的延长而增加，支持血吸虫获得性免疫发展缓慢的假设是由于对相关寄生虫抗原的反应性缓慢积累。
Background. This study compared patterns of recognition of defined Schistosoma haematobium adult worm antigens by serum antibodies from schistosome-exposed Zimbabweans aged 5-18 years.Methods. The population was stratified by age and infection intensity into 9 groups within which serum specimens were pooled and used to screen for protein recognition by 2-dimensional Western blotting. Recognized proteins were identified by electrospray ionizing tandem mass spectrometry.Results. A total of 71 antigens were recognized by >= 1 of the serum pools. The recognition varied distinctly with host age and infection intensity, with some isoform-specific responses. The repertoire of antigens recognized increased with age, peaking in the oldest participants whose had no or mild-to-moderate infection intensity. The intensity of antigen recognition also increased with age, peaking in the oldest participants with the heaviest infection intensity.Conclusions. The recognition of specific schistosome antigens, both in terms of the diversity of antigens recognized and the intensity of antigen recognition, increased with duration of exposure to infection, supporting the hypothesis that the slow development of schistosome-acquired immunity is due to the slow accumulation of responsiveness to relevant parasite antigens.