Studies on the Developmental Blocks in Cultured Hamster Embryos
Studies on the Developmental Blocks in Cultured Hamster Embryos
复制标题
培养仓鼠胚胎发育阻滞的研究
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
B. Bavister
中科院分区：
文献类型：
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作者：
B. Bavister
正如本书序言中所提到的，我们目前所知道的关于植入前胚胎发育调控的大多数知识都来自于体外培养条件下对小鼠胚胎的研究。这些信息具有巨大的价值，但我们应该关注的是，其他物种的关键数据相对缺乏（兔子是一个明显的例外，见第1章和第10章）。接受其他物种的胚胎需要深入研究，使用类似于过去20年左右应用于小鼠胚胎的实验方法，为什么选择金黄仓鼠作为模型物种？关于仓鼠胚胎培养的公开报道的稀缺性强调了体外培养这些胚胎的困难;事实上，从未有过成功培养仓鼠2细胞胚胎的报道。由于某些未知的原因，金黄仓鼠的早期卵裂期胚胎对常规培养条件格外敏感（与小鼠胚胎相比），并且“2细胞阻滞”似乎是绝对的（Yanagimachi和Chang，1964; Whittingham和Bavister，1974）。
As mentioned in the Preface to this book, most of what we presently know about the regulation of preimplantation embryo development is derived from studies with mouse embryos under in vitro culture conditions. This information is of enormous value, yet we should be concerned about the relative lack of critical data available for other species (with the notable exception of the rabbit: see Chapters 1 and 10). Accepting that embryos of other species need to be intensively investigated, using experimental approaches similar to those applied to mouse embryos over the past 20 years or so, why choose the golden hamster as a model species? The scarcity of published reports on the culture of hamster embryos underscores the difficulty of growing these embryos in vitro; in fact, there has never been a report of successful culture of hamster 2-cell embryos. For some unknown reason, early cleavage stage embryos of the golden hamster are extraordinarily sensitive (compared to mouse embryos) to conventional culture conditions, and the “2-cell block” appears to be absolute (Yanagimachi and Chang, 1964; Whittingham and Bavister, 1974).
影响因子：
6.6
作者：
G. Schatten;T. Bestor;R. Balczon;J. Henson;H. Schatten
通讯作者：
G. Schatten;T. Bestor;R. Balczon;J. Henson;H. Schatten