The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing.
The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing.
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DOI：
10.1083/jcb.127.3.609
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发表时间：
1994-11
期刊：
影响因子：
--
通讯作者：
Lee WH
中科院分区：
文献类型：
--
作者：
Durfee T;Mancini MA;Jones D;Elledge SJ;Lee WH
视网膜母细胞瘤蛋白（p110RB）的肿瘤抑制能力依赖于通过其羧基末端结构域与细胞蛋白质的相互作用。p110RB氨基末端区域如何促进这种活性尚不清楚，尽管现在有证据表明它对全长蛋白的生长抑制和调节都很重要。我们已经使用酵母双杂交系统来筛选与p110RB的前300个氨基酸结合的细胞蛋白。从这个屏幕上分离出的唯一的基因编码一种新的84 kD的核基质蛋白，定位于与RNA加工相关的亚核区域。这种蛋白质，p84，需要在p110RB的氨基末端的结构上确定的结构域进行结合。此外，在体内和体外实验表明，p84优先结合的功能活性，hypophosphorylated形式的p110RB。因此，p110 RB的氨基末端可能部分地起作用，以促进生长促进因子在亚核区域的结合，积极参与RNA代谢。
The tumor suppressing capacity of the retinoblastoma protein (p110RB) is dependent on interactions made with cellular proteins through its carboxy-terminal domains. How the p110RB amino-terminal region contributes to this activity is unclear, though evidence now indicates it is important for both growth suppression and regulation of the full- length protein. We have used the yeast two-hybrid system to screen for cellular proteins which bind to the first 300 amino acids of p110RB. The only gene isolated from this screen encodes a novel 84-kD nuclear matrix protein that localizes to subnuclear regions associated with RNA processing. This protein, p84, requires a structurally defined domain in the amino terminus of p110RB for binding. Furthermore, both in vivo and in vitro experiments demonstrate that p84 binds preferentially to the functionally active, hypophosphorylated form of p110RB. Thus, the amino terminus of p110RB may function in part to facilitate the binding of growth promoting factors at subnuclear regions actively involved in RNA metabolism.