CELL PROLIFERATION IN NEURAL TUBE - ELECTRON MICROSCOPIC AND GOLGI ANALYSIS IN MOUSE CEREBRAL VESICLE
CELL PROLIFERATION IN NEURAL TUBE - ELECTRON MICROSCOPIC AND GOLGI ANALYSIS IN MOUSE CEREBRAL VESICLE
复制标题
DOI：
10.1007/bf00391127
复制
发表时间：
1971-01-01
期刊：
影响因子：
--
通讯作者：
RUFFETT, TL
中科院分区：
文献类型：
--
作者：
HINDS, JW;RUFFETT, TL
研究了心室（原始室管膜）细胞在其发生周期中的形态和精细结构。间期细胞是径向定向的双极元件，其突起跨越脑壁的厚度。在心室连接内部突起的小带连接复合体由缝隙连接和更宽的中间连接组成。外界层由简单并置的扩张端足组成;它们类似于轴突生长锥，含有60 μ m的微丝，光滑内质网的元素，但没有微管。在前期，心室细胞的核移动到一个近心室的位置，而它们的外部过程保持充分延伸。这些细胞的内部突起含有许多纵向排列的微管和微丝。继核迁移之后，在前中期或中期，细胞退出或夹断其外突，变成近球形。在分裂末期，不对称的沟形成导致子细胞之间的薄连接体（中间体），其邻近心室并通过连接复合体附着在那里。在完全分离之前或之后，外部过程开始朝向外部限制层重新生长，最终再次导致双极相间电池。微丝存在于外突长出之前的末期细胞和外突的生长尖端。
The shape and fine structure of ventricular (primitive ependymal) cells during their generation cycle was studied. Interphase cells are radially oriented bipolar elements with processes spanning the thickness of the brain wall. Zonular junctional complexes joining internal processes at the ventricle consist of gap junctions and wider intermediate junctions. The external limiting layer consists of expanded end-feet in simple apposition; they resemble axonal growth cones and contain a feltwork of 60 Å microfilaments, elements of smooth endoplasmic reticulum but no microtubules. During prophase, nuclei of ventricular cells move to a juxtaventricular position, while their external processes remain fully extended. The internal processes of such cells contain numerous longitudinally arranged microtubules and microfilaments. Subsequent to nuclear migration, in prometaphase or metaphase, the cell withdraws or pinches off its external process and becomes nearly spherical. During telophase an asymmetrical furrow formation results in a thin connector (midbody) between daughter cells which is adjacent to the ventricle and attached there by the junctional complex. Either before or after complete separation, an external process starts regrowing towards the external limiting layer, eventually resulting in a bipolar interphase cell again. Microfilaments are present in telophase cells before outgrowth of external processes and in growing tips of external processes.