NB4 cells treated with all-trans retinoic acid generate toxic reactive oxygen species that cause endothelial hyperpermeability
NB4 cells treated with all-trans retinoic acid generate toxic reactive oxygen species that cause endothelial hyperpermeability
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DOI：
10.1016/j.leukres.2009.05.022
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发表时间：
2010-03-01
影响因子：
2.7
通讯作者：
Uchiyama, Takashi
中科院分区：
文献类型：
--
作者：
Miyoshi, Takashi;Arai, Toshiyuki;Uchiyama, Takashi
维甲酸综合征（RAS）是急性早幼粒细胞白血病患者在全反式维甲酸（RA）诱导治疗过程中的严重并发症。在这项研究中，我们研究了活性氧（ROS）是否参与毛细血管渗漏现象在RAS中，使用NB 4细胞。当细胞被佛波醇12-肉豆蔻酸酯13-乙酸酯刺激时，具有成熟髓过氧化物酶的RA处理的细胞产生毒性ROS，如单线态氧、次氯酸和羟基自由基，并引起内皮通透性增高。来自患者的白血病细胞也产生毒性ROS。这些结果表明，有毒的活性氧有助于RAS毛细血管渗漏现象的发展。(C)2009爱思唯尔有限公司保留所有权利。
Retinoic acid syndrome (RAS) is a serious complication during induction therapy with all-trans retinoic acid (RA) in patients with acute promyelocytic leukemia. In this study, we examined whether reactive oxygen species (ROS) were involved in capillary leak phenomenon in RAS, using NB4 cells. When cells were stimulated by phorbol 12-myristate 13-acetate, RA-treated cells with matured myeloperoxidase produced toxic ROS, such as singlet oxygen, hypochlorous acid and hydroxyl radical, and brought about endothelial hyperpermeability. Leukemic cells from a patient also produced toxic ROS. These findings indicated that toxic ROS contribute to the development of capillary leak phenomenon in RAS. (C) 2009 Elsevier Ltd. All rights reserved.