A novel carbohydrate structure in bovine and ovine luteinizing hormones.
A novel carbohydrate structure in bovine and ovine luteinizing hormones.
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牛和羊促黄体激素中的新型碳水化合物结构。
DOI：
10.1016/0006-291x(80)90078-9
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发表时间：
1980
影响因子：
3.1
通讯作者：
Bedi,GS
中科院分区：
文献类型：
--
作者：
Bahl,OP;Reddy,MS;Bedi,GS
牛和绵羊促黄体激素（bLH 和 oLH）具有三个相似的天冬酰胺连接碳水化合物单元，由 Fuc、Gal（仅存在于 oLH）、Man、GlcNAc 和 GalNAc 组成。对通过碱性硼氢化物处理激素和天然激素获得的寡糖进行了这些碳水化合物单元的结构分析。通过甲基化、高碘酸盐氧化和脱氨技术以及外切糖苷酶处理来测定糖间和异头连接、单糖序列和多肽-碳水化合物连接。基于这些研究，提出了 bLH 和 oLH 寡糖的结构。
Bovine and ovine lutropins (bLH and oLH) have three similar asparagine-linked carbohydrate units made up of Fuc, Gal (present only in oLH), Man, GlcNAc and GalNAc. The structural analyses of these carbohydrate units were performed on the oligosaccharides obtained by the alkaline borohydride treatment of the hormones and on the native hormones. The determination of intersugar and anomeric linkages, monosaccharide sequences and the polypeptide-carbohydrate linkage was carried out by methylation, periodate oxidation and deamination techniques and treatment with exoglycosidases. Based on these studies the structure for the oligosaccharide of bLH and oLH is proposed.