The disulfide bond pairing of the pheromones Er-1 and Er-2 of the ciliated protozoan Euplotes raikovi.
The disulfide bond pairing of the pheromones Er-1 and Er-2 of the ciliated protozoan Euplotes raikovi.
复制标题
纤毛原生动物 Euplotes raikovi 信息素 Er-1 和 Er-2 的二硫键配对。
DOI：
10.1002/pro.5560010609
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Bradshaw,RA
中科院分区：
文献类型：
--
作者：
Stewart,AE;Raffioni,S;Chaudhary,T;Chait,BT;Luporini,P;Bradshaw,RA
两个Euplotes raikovipheromones Er-1 和Er-2 的二硫键配对已通过化学和质谱分析确定。通过反相高效液相色谱纯化来自天然分子热解消化的胱氨酸连接肽，并在已知序列中进行鉴定以进行分配。在两种信息素中都发现了相同的配对，Cys(I)–Cys(IV)、Cys(II)–Cys(VI) 和 Cys(III)–Cys(V)，表明这种模式在该分子家族中普遍存在。二硫化物的这种排列表明三维结构由三个环定义，从一种信息素到另一种信息素，这些环的大小和电荷分布可能不同。
The disulfide pairings of the twoEuplotes raikovipheromones Er‐1 and Er‐2 have been determined by chemical and mass spectrometric analyses. Cystine‐linked peptides from thermolytic digestions of the native molecules were purified by reverse‐phase high performance liquid chromatography and identified in the known sequences to make the assignments. The same pairing, Cys(I)–Cys(IV), Cys(II)–Cys(VI), and Cys(III)–Cys(V), was found in both pheromones, suggesting that this pattern occurs commonly throughout this family of molecules. This arrangement of disulfides indicates that the three‐dimensional structure is defined by three loops, which can vary in size and charge distribution from one pheromone to another.