Developmental change in TATA-box utilization during preimplantation mouse development
Developmental change in TATA-box utilization during preimplantation mouse development
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DOI：
10.1006/dbio.1999.9486
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发表时间：
2000-02-15
影响因子：
2.7
通讯作者：
Schultz, RM
中科院分区：
文献类型：
--
作者：
Davis, W;Schultz, RM
胚胎基因组在植入前小鼠发育期间的激活以对进一步发育至关重要的基因表达的显着重新编程为特征。蛋白质翻译起始因子eIF-1a基因的表达由近端含有TATA的启动子和远端不含TATA的启动子驱动。通过对来自不含TATA启动子和含TATA启动子的转录本的特异扩增，我们发现70%的EIF-1A转录本来自发育成熟的卵母细胞中含有TATA启动子的转录本。胚胎基因组在两细胞阶段的激活伴随着启动子利用的变化，以至于现在只有25%的转录本来自含TATA的启动子，即75%来自不含TATA的启动子。当单细胞胚胎在α-Amanitin存在下培养到两细胞阶段时，转录物丰度的这种变化没有观察到，即转录物的分布与在卵母细胞中观察到的相似。到囊胚阶段，只有5%的转录本是由含有TATA的启动子产生的。如果EIF-1A的TATA盒利用的变化反映了TATA盒利用的潜在的全球变化，那么在植入前的发育过程中，启动子的利用可能会发生戏剧性的变化，从而使TATA较少的启动子得到更有效的利用。启动子利用的这种变化可能大大有助于母体向合子转变过程中发生的基因表达的重新编程，(C)2000学术出版社。
Activation of the embryonic genome during preimplantation mouse development is characterized by a marked reprogramming of gene expression that is essential for further development. Expression of the protein translation initiation factor eIF-1A gene is driven by a proximal TATA-containing promoter and a distal TATA-less promoter. Using specific amplification of cDNA ends that resolves transcripts derived from the TATA-less and TATA-containing promoters, we find that 70% of the eIF-1A transcripts are derived from the TATA-containing promoter in the fully-grown oocyte. Activation of the embryonic genome during the two-cell stage is accompanied by a change in promoter utilization such that only 25% of the transcripts are now derived from the TATA-containing promoter, i.e., 75% are derived from the TATA-less promoter. When one-cell embryos are cultured to the two-cell stage in the presence of alpha-amanitin, this change in transcript abundance is not observed, i.e., the distribution of transcripts is similar to that observed in the oocyte. By the blastocyst stage only 5% of the transcripts are generated from the TATA-containing promoter. If the change in TATA-box utilization for the eIF-1A reflects an underlying global change in TATA-box utilization, a dramatic change in promoter utilization may occur during preimplantation development such that TATA-less promoters are more efficiently utilized. Such a change in promoter utilization could contribute significantly to the reprogramming of gene expression that occurs during the maternal-to-zygotic transition, (C) 2000 Academic Press.