Liquiritin potentiate neurite outgrowth induced by nerve growth factor in PC12 cells
Liquiritin potentiate neurite outgrowth induced by nerve growth factor in PC12 cells
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DOI：
10.1007/s10616-009-9226-8
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发表时间：
2009-07-01
期刊：
影响因子：
2.2
通讯作者：
Bian, Guang-Xing
中科院分区：
文献类型：
--
作者：
Chen, Zheng-ai;Wang, Jun-Long;Bian, Guang-Xing
神经突起的生长和神经元的分化在神经系统的发育中起着至关重要的作用。了解神经营养因子诱导的轴突生长对于制定神经退行性疾病以及各种神经损伤后轴突再生的治疗策略具有重要意义。有报道称，神经生长因子(NGF)对PC12细胞突起的伸展和交感神经元样表型的分化具有调节作用。在本研究中，我们观察了NGF介导的PC12细胞在甘草酸作用下的突起生长。甘草苷是从甘草中提取的一种黄酮类化合物，在传统的东方医学中，由于其具有增强生命和解毒的作用，经常被用于治疗损伤或肿胀。结果表明，甘草苷对NGF刺激的PC12细胞突起生长有明显的促进作用，且呈剂量依赖关系，而甘草苷单独作用不能诱导PC12细胞突起生长。寡核苷酸芯片和RT-PCR分析进一步阐明甘草素的神经营养作用与神经原蛋白3、神经纤维瘤病1、Noch基因同源物2、神经营养素U受体2和神经营养素5等神经相关基因的过度表达有关。因此，甘草素可能是治疗阿尔茨海默病和帕金森病等多种神经退行性疾病的良好候选药物。
Neurite outgrowth and neuronal differentiation play a crucial role in the development of the nervous system. Understanding of neurotrophins induced neurite outgrowth was important to develop therapeutic strategy for axon regeneration in neurodegenerative diseases as well as after various nerve injuries. It has been reported that extension of neurite and differentiation of sympathetic neuron-like phenotype was modulated by nerve growth factor (NGF) in PC12 cells. In this study, NGF mediated neurite outgrowth was investigated in PC12 cells after liquiritin exposure. Liquiritin is a kind of flavonoids that is extracted from Glycyrrhizae radix, which is frequently used to treat injury or swelling for its life-enhancing properties as well as detoxification in traditional Oriental medicine. The result showed that liquiritin significantly promotes the neurite outgrowth stimulated by NGF in PC12 cells in dose dependant manners whereas the liquiritin alone did not induce neurite outgrowth. Oligo microarray and RT-PCR analysis further clarified that the neurotrophic effect of liquiritin was related to the overexpression of neural related genes such as neurogenin 3, neurofibromatosis 1, notch gene homolog 2, neuromedin U receptor 2 and neurotrophin 5. Thus, liquiritin may be a good candidate for treatment of various neurodegenerative diseases such as Alzheimer's disease or Parkinson's disease.