First steps towards a rational use of African rice, Oryza glaberrima, in rice breeding through a 'contig line' concept
First steps towards a rational use of African rice, Oryza glaberrima, in rice breeding through a 'contig line' concept
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DOI：
10.1023/a:1003045518236
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发表时间：
1997-01-01
期刊：
影响因子：
1.9
通讯作者：
Lorieux, M
中科院分区：
文献类型：
--
作者：
Ghesquiere, A;Sequier, J;Lorieux, M
过去，非洲栽培稻Oryza glaberrima在O. Sativa育种受到种间F-1和早期后代高度不育的阻碍。最近开发的映射分子标记提供了合理化和监测两个栽培稻物种之间的基因渗入的机会。通过种间杂交和回交开发这样的渐渗系代表了创造新的遗传和超亲变异的高潜力。我们正在启动一个原始的标记辅助回交程序，目的是产生一组100个“重叠群品系”，每个品系都带有一个外来O。glaberrima染色体片段约20 cM的O. sativa遗传背景作为初步的研究，我们正在建立一个基于STS和RAPD标记的遗传连锁图谱。sativa和O. glaberrima。然后选择BC 1植物以加速仅显示靶片段的“重叠群系”的产生。预计种间多态性足够大，使这种方法可行。该方法具有以下优点：（1）系统评价O. glaberrima，（ii）改进和简化的QTL检测和（iii）比较分析的基因组组织。glaberrima vs. sativa.本文介绍了利用STS和RAPD技术对亲本间的多态性进行评价和对BC 1群体的发展进行研究的现状。
In the past, utilisation of African cultivated rice, Oryza glaberrima, in O. sativa breeding was hampered by high sterility in interspecific F-1 and in early progenies. Recent development of mapped molecular markers provides the opportunity to rationalise and to monitor introgressions between the two cultivated rice species. Development of such introgressed lines through interspecific hybridisation and backcrossing represents high potential to create new genetic and transgressive variation. We are initiating an original marker-aided backcross program with the aim of producing a set of 100 'contig lines', each one bearing an alien O. glaberrima chromosomal fragment of around 20 cM in the O. sativa genetic background. As a preliminary step, we are now developing a genetic linkage map based on STS and RAPD markers, using an interspecific backcross between O. sativa and O. glaberrima. BC1 plants will then be selected to expedite the production of 'contig lines' exhibiting only the target fragment. The interspecific polymorphism is anticipated to be large enough to make this methodology feasible. Many advantages are expected from this approach, such as: (i) systematic assessment of the useful genetic resources displayed by O. glaberrima, (ii) improved and simplified detection of QTLs and (iii) comparative analysis of genomic organisation of O. glaberrima vs. O. sativa. Current work on evaluation of STS and RAPD polymorphism between parents and development of the BC1 population is presented.