Long-term nitrogen and phosphorus fertilization reveals that phosphorus limitation shapes the microbial community composition and functions in tropical montane forest soil
Long-term nitrogen and phosphorus fertilization reveals that phosphorus limitation shapes the microbial community composition and functions in tropical montane forest soil
复制标题
长期氮磷施肥揭示磷限制影响热带山地森林土壤微生物群落的组成和功能
DOI：
10.1016/j.scitotenv.2022.158709
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发表时间：
2023
影响因子：
9.8
通讯作者：
Yakov Kuzyakov
中科院分区：
文献类型：
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作者：
Xiaomin Ma;Zhang Zhou;Jie Chen;Han Xu;Suhui Ma;Michaela A. Dippold;Yakov Kuzyakov
微生物控制着土壤养分循环。因此，了解它们对人类引起的N和P输入增加的反应至关重要。我们调查了微生物群落组成，生物量，功能基因丰度和酶活性，以响应10年的N和P添加在一个主要的热带山地森林，我们探讨了这些影响背后的驱动因素。真菌比细菌对养分添加更敏感，真菌群落的变化主要受磷的有效性驱动。氮的添加加剧了磷的限制，微生物通过增加磷循环功能基因的丰度和磷酸酶活性来响应。与此相反，磷的添加缓解了磷缺乏，从而降低了磷循环功能基因的丰度和磷酸酶活性。土壤微生物群落组成的变化、参与磷循环的功能基因的变化以及磷酸酶活性的变化主要是由施磷引起的，同时也引起了土壤化学计量（C/P和N/P）的改变。通过施肥消除缺磷，通过提高碳降解酶的活性来加速碳循环。碳、磷功能基因丰度呈正相关，表明磷限制森林土壤中碳、磷循环存在强烈耦合。总之，长期施肥试验表明，土壤微生物可以适应环境引起的土壤养分化学计量的变化，不仅通过改变微生物群落组成和功能基因丰度，而且还通过调节酶的产生。微生物群落对氮磷失衡的响应以及微生物群落对土壤养分循环的影响应纳入生态系统生态地球化学模型。
Microorganisms govern soil nutrient cycling. It is therefore critical to understand their responses to human-induced increases in N and P inputs. We investigated microbial community composition, biomass, functional gene abundance, and enzyme activities in response to 10-year N and P addition in a primary tropical montane forest, and we explored the drivers behind these effects. Fungi were more sensitive to nutrient addition than bacteria, and the fungal community shift was mainly driven by P availability. N addition aggravated P limitation, to which microbes responded by increasing the abundance of P cycling functional genes and phosphatase activity. In contrast, P addition alleviated P deficiency, and thus P cycling functional gene abundance and phosphatase activity decreased. The shift of microbial community composition, changes in functional genes involved in P cycling, and phosphatase activity were mainly driven by P addition, which also induced the alteration of soil stoichiometry (C/P and N/P). Eliminating P deficiency through fertilization accelerated C cycling by increasing the activity of C degradation enzymes. The abundances of C and P functional genes were positively correlated, indicating the intensive coupling of C and P cycling in P-limited forest soil. In summary, a long-term fertilization experiment demonstrated that soil microorganisms could adapt to induced environmental changes in soil nutrient stoichiometry, not only through shifts of microbial community composition and functional gene abundances, but also through the regulation of enzyme production. The response of the microbial community to N and P imbalance and effects of the microbial community on soil nutrient cycling should be incorporated into the ecosystem biogeochemical model.