Identification and gene expression of multiple peptidoglycan recognition proteins (PGRPs) in the deep-sea mussel Bathymodiolus azoricus, involvement in symbiosis?
Identification and gene expression of multiple peptidoglycan recognition proteins (PGRPs) in the deep-sea mussel Bathymodiolus azoricus, involvement in symbiosis?
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DOI：
10.1016/j.cbpb.2017.02.002
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发表时间：
2017-05-01
影响因子：
2.2
通讯作者：
Mary, Jean
中科院分区：
文献类型：
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作者：
Detree, Camille;Lallier, Francois H.;Mary, Jean
深海贻贝Bathymodiolus azoricus 与其硫营养（SOX）和甲烷营养（MOX）共生体之间的关系已在生态和功能上得到了深入研究。内共生在深海热液喷口动物群中很常见，但人们对宿主与共生体之间相互作用调节的分子机制知之甚少。在这项研究中，我们重点关注一组称为 PGRP 的模式识别受体 (PRR)，它们能够识别细菌细胞壁的肽聚糖。我们首先对 B. azoricus 鳃中不同的 PGRP 亚型进行了表征，并鉴定了 5 个旁系同源物。其中两个显示出信号肽。然后，使用为每个旁系同源物设计的特定探针对因原位实验程序而显示出各种细菌含量的个体的鳃进行实时PCR定量。总体而言，我们发现当共生体数量减少时，PGRP 表达减少，这表明 PGRP 在 B. azoricus 鳃中共生体的调节中发挥着作用。因此，我们假设分泌蛋白可以作为合作信号来诱导共生组织的定植，而非分泌蛋白可以调节鳃组织内内共生体的密度。 (C) 2017 Elsevier Inc. 保留所有权利。
The relationship between the deep-sea mussel Bathymodiolus azoricus and its thiotrophic (SOX) and methanotrophic (MOX) symbionts has been ecologically and functionally well studied. Endosymbiosis is common in deep-sea hydrothermal vent fauna, yet little is known about the molecular mechanisms underlying the regulation of interactions between host and symbionts. In this study we focused on a group of pattern recognition receptors (PRR), called PGRPs that are able to recognize the peptidoglycan of bacterial cell wall. We first characterised the different PGRPs isoforms in B. azoricus gills and identified five paralogs. Among them two displayed a signal peptide. Then, specific probes designed for each paralog were used to perform real-time PCR quantification in gills of individuals showing various bacterial content as a result of in situ experimental procedures. Overall we found a decrease of PGRPs expression when symbionts amount decreases, suggesting an implication of PGRPs in the regulation of symbionts in B. azoricus gills. We therefore hypothesize that secreted proteins could act as cooperation signals to induce colonisation of symbiotic tissue while non-secreted proteins may regulate the density of endosymbionts within the gill tissue. (C) 2017 Elsevier Inc. All rights reserved.