Carbon partitioning and the impact of starch deficiency on the initial response of Arabidopsis to chilling temperatures
Carbon partitioning and the impact of starch deficiency on the initial response of Arabidopsis to chilling temperatures
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DOI：
10.1016/j.plantsci.2011.05.005
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发表时间：
2011-08-01
期刊：
影响因子：
5.2
通讯作者：
Sicher, Richard
中科院分区：
文献类型：
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作者：
Sicher, Richard
拟南芥的代谢产物和逆境相关的转录产物被检测到对低温的响应。冷处理的碳同化率平均提高了17%。蔗糖、葡萄糖和果糖的积累消耗了来自A的碳的42%，而叶淀粉只能合成接近观察到的可溶性糖变化的10%。碳水化合物是在冷处理的前24小时内积累的唯一主要代谢物。除麦芽糖和棉籽糖外，其他碳水化合物的积累在黑暗条件下被取消。淀粉水解与麦芽糖积累和BAM3表达增加有关，BAM3编码淀粉动员所必需的β-淀粉酶。在无淀粉(Pgm1)突变体中，己糖的积累延迟了6h，棉子糖的积累没有观察到。在野生型和pgrn1突变体中，5个逆境诱导的转录本的表达变化与野生型相似。与光照相比，在黑暗中进行冷处理时，五个与应激相关的转录本中有三个的表达减少。因此，在冷休克后的第一个24小时内，淀粉水解会增加己糖和棉子糖的积累，在黑暗中的冷处理中，拟南芥观察到了部分的冷胁迫反应。爱思唯尔爱尔兰有限公司出版。
Metabolites and stress related transcripts were measured in Arabidopsis thaliana in response to chilling temperatures. Rates of carbon assimilation increased 17% on average in response to cold treatment. Sucrose, glucose and fructose accumulation consumed 42% of the carbon from A but leaf starch only could synthesize similar to 10% of observed changes in soluble sugars. Carbohydrates were the only major class of metabolites that accumulated during the first 24 h of cold treatment. Except maltose and raffinose, carbohydrate accumulation was abolished when cold treatments were in darkness. Starch hydrolysis was correlated with maltose accumulation and increased expression of BAM3, which encodes a beta-amylase necessary for starch mobilization. Hexose accumulation was delayed 6 h and raffinose accumulation was not observed in a starchless (pgm1) mutant. Changes of expression of five stress-induced transcripts in response to cold were similar in the wild type and in the pgrn1 mutant. Three of five stress related transcripts had decreased expression when cold treatments were performed in the dark compared to the light. Therefore, starch hydrolysis may augment hexose and raffinose accumulations during the first 24 h after a cold shock and a partial cold stress response was observed in Arabidopsis during cold treatments in the dark. Published by Elsevier Ireland Ltd.