Identification of testis‐specific ubiquitin‐conjugating enzyme in the ascidian Ciona intestinalis
Identification of testis‐specific ubiquitin‐conjugating enzyme in the ascidian Ciona intestinalis
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DOI：
10.1002/mrd.21198
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发表时间：
2010-07
影响因子：
2.5
通讯作者：
Naoto Yokota;Y. Harada;H. Sawada
中科院分区：
文献类型：
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作者：
Naoto Yokota;Y. Harada;H. Sawada
众所周知，泛素-蛋白酶体系统在海鞘、海胆和哺乳动物的受精过程中发挥着关键作用。为了深入了解生殖系统中涉及的泛素结合酶 (Ube2)，我们系统地探索了海鞘海鞘睾丸中表达的 Ube2 酶。在这里，我们报告了一种新型 Ube2r (Ci0100152677) 的 cDNA 克隆和表征，该 Ube2r 能够与泛素形成硫醇酯键。 Northern分析、整体原位杂交和免疫细胞化学表明，该酶仅在睾丸中表达，主要在精子发生后期的生殖细胞中表达，并且定位于精子头部和尾部，表明该酶可能参与受精或精子发生/精子发生。摩尔。重现。开发。 77: 640–647, 2010。© 2010 Wiley-Liss, Inc.
The ubiquitin–proteasome system is known to play a key role in fertilization in ascidians, sea urchins, and mammals. To obtain insights into the ubiquitin‐conjugating enzymes (Ube2) involved in reproductive systems, we systematically explored Ube2 enzymes expressed in the testis of the ascidian Ciona intestinalis. Here, we report cDNA cloning and characterization of a novel type of Ube2r (Ci0100152677) that is capable of making a thiolester bond with ubiquitin. Northern analysis, whole‐mount in situ hybridization and immunocytochemistry indicate that this enzyme is exclusively expressed in the testis, mainly in the germ cells during the late stage of spermatogenesis, and is localized in the sperm head and tail, suggesting possible participation in fertilization or spermatogenesis/spermiogenesis. Mol. Reprod. Dev. 77: 640–647, 2010. © 2010 Wiley‐Liss, Inc.