Effects of seasonal variation on the central nervous system activity of Ocimum gratissimum L. essential oil
Effects of seasonal variation on the central nervous system activity of Ocimum gratissimum L. essential oil
复制标题
DOI：
10.1016/j.jep.2005.10.013
复制
发表时间：
2006-04-21
影响因子：
5.4
通讯作者：
Costa, M
中科院分区：
文献类型：
--
作者：
Freire, CMM;Marques, MOM;Costa, M
茴香（Lamiaceae）和同属的其他物种被用作治疗世界温暖地区常见的中枢神经系统（CNS）疾病的药物。茴香精油的化学成分根据其化学类型而变化：时间酚、丁香酚或香叶醇。本研究采用加氢蒸馏法在一年四季提取精油型丁香酚。在露天和旋转杆试验中评估了对中枢神经系统的活性；测定戊巴比妥钠（PBS, 40 mg/kg，腹腔注射）诱导的睡眠时间和戊四唑（PTZ, 85 mg/kg, s.c）和最大电休克（MES, 50 mA, 0.11 s）诱导的癫痫发作的抗惊厥活性。每个季节获得的精油都能有效地延长睡眠时间，春天获得的一种制剂能够保护动物免受电击引起的强直性癫痫发作。在不同季节，丁香酚和1,8-桉树脑的含量最多，而在春季，倍半萜类化合物的相对含量最高，说明这些化合物可以解释一年中不同季节精油生物活性的差异。2005爱思唯尔爱尔兰有限公司版权所有。
Ocimum gratissimum L. (Lamiaceae) and other species of the same genus are used as medicines to treat central nervous system (CNS) diseases, commonly encountered in warm regions of the world. The chemical composition of Ocimum gratissimum essential oil varies according to their chemotypes: timol, eugenol or geraniol. In this study, the essential oil type eugenol was extracted by hydrodistillation in each of the four seasons of the year. Activity upon CNS was evaluated in the open-field and rota-rod tests; sleeping time induced by sodium pentobarbital (PBS, 40 mg/kg, intraperitoneally, i.p.) and anticonvulsant activity against seizures induced by both pentylenetetrazole (PTZ; 85 mg/kg, s.c.) and maximal electroshock (MES, 50 mA, 0.11 s) were determined. Essential oils obtained in each season were effective in increasing the sleeping duration and a preparation obtained in Spring was able to protect animals against tonic seizures induced by electroshock. In each season, eugenol and 1,8-cineole were the most abundant compounds, and in Spring the essential oil presented the greatest relative percentage of sesquiterpenes, suggesting that these compounds could explain the differences observed in the biological activity in essential oils obtained in different seasons of the year. (c) 2005 Elsevier Ireland Ltd. All rights reserved.