Measurements of Retinal Perfusion Using Laser Speckle Flowgraphy and Doppler Optical Coherence Tomography
Measurements of Retinal Perfusion Using Laser Speckle Flowgraphy and Doppler Optical Coherence Tomography
复制标题
DOI：
10.1167/iovs.16-19896
复制
发表时间：
2016-10-01
影响因子：
4.4
通讯作者：
Schmetterer, Leopold
中科院分区：
文献类型：
--
作者：
Luft, Nikolaus;Wozniak, Piotr A.;Schmetterer, Leopold
目的。本研究通过体外实验并与健康白种人群体中的双光束多普勒光学相干断层扫描 (D-OCT) 直接比较，评估了使用激光散斑血流成像 (LSFG) 进行视网膜灌注测量的有效性。方法。使用 LSFG 在 17 种不同的流速（范围，0.5-47 mm/s）下测量通过玻璃毛细管泵送的散射溶液的流速。玻璃毛细管内的流动由计算机控制的输液泵产生。在体内，在药物扩瞳前后对 20 名健康白人受试者的 20 只眼睛进行了 3 次连续的 LSFG 扫描。相对流量 (RFV)（LSFG 的主要输出参数）相对于从 D-OCT 获得的视网膜血流和速度的绝对测量值进行了比较验证。结果。在体外实验中，发现 RFV 在大约 700 任意单位 (au) 或实际速度 23.5 mm/s 的水平处饱和。在体内，RFV 与动脉 (r = 0.69，P = 0.001) 和静脉 (r = 0.74，P < 0.001) 中 D-OCT 获得的绝对血流测量结果显着一致。然而，线性回归分析显示，动脉和静脉中 RFV 的显着正零偏移值分别为 223.4 和 282.7 au。结论。 RFV 的测量可成功获得、可重复且不受药理瞳孔扩张的影响。然而，我们的数据揭示了测量白种人视网膜灌注的 LSFG 方法的缺陷。需要对该技术进行调整来解决 RFV 的明显问题，特别是动脉流速较高时的饱和效应。目前的数据集可能为此提供一个有价值的工具。 （临床试验。政府编号 NCT02582411）。
PURPOSE. This study evaluated the validity of retinal perfusion measurements using laser speckle flowgraphy (LSFG) by means of in vitro experiments and direct comparison with dual-beam Doppler optical coherence tomography (D-OCT) in a healthy Caucasian population.METHODS. The flow velocity of scattering solution pumped through a glass capillary was measured at 17 different flow velocities (range, 0.5-47 mm/s) using LSFG. The flow within the glass capillary was produced by a computer-controlled infusion pump. In vivo, three consecutive LSFG scans were obtained in 20 eyes of 20 healthy Caucasian subjects before and after pharmacological pupil dilation. Relative flow volume (RFV), the primary output parameter of LSFG, was comparatively validated relative to absolute measurements of retinal blood flow and velocity as obtained from D-OCT.RESULTS. In the in vitro experiments, RFV was found to saturate at a level of approximately 700 arbitrary units (au) or 23.5 mm/s of actual velocity. In vivo, RFV was in significant agreement with absolute blood flow measurements as obtained from D-OCT in arteries (r = 0.69, P = 0.001) and veins (r = 0.74, P < 0.001). However, linear regression analysis revealed significant positive zero offset values for RFV of 223.4 and 282.7 au in arteries and veins, respectively.CONCLUSIONS. Measurements of RFV were successfully obtainable, reproducible, and not influenced by pharmacological pupil dilation. Nevertheless, our data revealed flaws in the LSFG method of measuring retinal perfusion in Caucasians. Adjustment to the technique is required to address apparent issues with RFV, especially saturation effects with higher arterial flow rates. The present dataset may provide a valuable tool to do so. (Clinicaltrials. gov number NCT02582411).