Intermediates of yeast meiotic recombination contain heteroduplex DNA
Intermediates of yeast meiotic recombination contain heteroduplex DNA
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DOI：
10.1016/s1097-2765(01)00280-5
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发表时间：
2001-07-01
期刊：
影响因子：
16
通讯作者：
Lichten, M
中科院分区：
文献类型：
--
作者：
Allers, T;Lichten, M
异源双链DNA的形成在同源重组的所有模型中都是显著的特征。目前的双链断裂修复模型中的一个中心中间体包含两个Holliday连接，位于异源双链DNA区域的两侧。对酵母减数分裂的研究已经发现了这种中间体，但未能检测到相关的异源双链DNA。我们在这里表明，这些中间体含有异源双链DNA，为双链断裂修复模型提供了重要的验证。然而，我们也检测到中间产物，其中两个Holliday连接都在起始DSB位点的一侧，而中间区域没有显示出异源双链DNA的证据。这种结构不容易被双链断裂修复模型的规范版本所适应。
The formation of heteroduplex DNA features prominently in all models for homologous recombination. A central intermediate in the current double-strand break repair model contains two Holliday junctions flanking a region of heteroduplex DNA. Studies of yeast meiosis have identified such intermediates but failed to detect associated heteroduplex DNA. We show here that these intermediates contain heteroduplex DNA, providing an important validation of the double-strand break repair model. However, we also detect intermediates where both Holliday junctions are to one side of the initiating DSB site, while the intervening region shows no evidence of heteroduplex DNA. Such structures are not easily accommodated by the canonical version of the double-strand break repair model.